ArticleJournal of cellular and molecular medicine2009
Platelet lysate promotes in vitro wound scratch closure of human dermal fibroblasts: different roles of cell calcium, P38, ERK and PI3K/AKT.
Article in Journal of cellular and molecular medicine, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 50 citations in OpenAlex.
- Umbilical Cord PRP Accelerates Corneal Wound Healing via AQP1 Upregulation and Calcium Signaling.Biology · 2026Article
- Human platelet lysate: a potential therapeutic for intracerebral hemorrhage.Frontiers in neuroscience · 2024Review
- Single step method for high yield human platelet lysate production.Transfusion · 2023Article
- Assessment of factors affecting flicker ERGs recorded with RETeval from data obtained from health checkup screening.PloS one · 2023Article
- Bone-targeting delivery of platelet lysate exosomes ameliorates glucocorticoid-induced osteoporosis by enhancing bone-vessel coupling.Journal of nanobiotechnology · 2022Article
- Developing a Glyoxal-Crosslinked Chitosan/Gelatin Hydrogel for Sustained Release of Human Platelet Lysate to Promote Tissue Regeneration.International journal of molecular sciences · 2021Article
- Article
- Mechanical stretch promotes hypertrophic scar formation through mechanically activated cation channel Piezo1.Cell death & disease · 2021Article
- The Lack of a Representative Tendinopathy Model Hampers Fundamental Mesenchymal Stem Cell Research.Frontiers in cell and developmental biology · 2021Review
- Platelet lysate for COVID-19 pneumonia-a newer adjunctive therapeutic avenue.Stem cell investigation · 2021Review
- Defined serum-free three-dimensional culture of umbilical cord-derived mesenchymal stem cells yields exosomes that promote fibroblast proliferation and migration in vitro.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2021Article
- Platelet-derived transforming growth factor-β1 promotes keratinocyte proliferation in cutaneous wound healing.Journal of tissue engineering and regenerative medicine · 2020Article
- Endothelial response boosted by platelet lysate: the involvement of calcium toolkit.International journal of molecular sciences · 2020Article
- Comparison of the efficacy of cryopreserved human platelet lysate and refrigerated lyophilized human platelet lysate for wound healing.Regenerative therapy · 2019Article
- Propolis Induces AQP3 Expression: A Possible Way of Action in Wound Healing.Molecules (Basel, Switzerland) · 2019Article
- Honey-Mediated Wound Healing: H₂O₂ Entry through AQP3 Determines Extracellular CaInternational journal of molecular sciences · 2019Article
- Comparative Analysis of Different Platelet Lysates and Platelet Rich Preparations to Stimulate Tendon Cell Biology: An In Vitro Study.International journal of molecular sciences · 2018Article
- Honeydew honey: biological effects on skin cells.Molecular and cellular biochemistry · 2017Article
- A Bench-TopApplied in vitro toxicology · 2016Article
- Highly EfficientStem cells international · 2016Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
There is a growing interest for the clinical use of platelet derivates in wound dressing. Platelet beneficial effect is attributed to the release of growth factors and other bioactive substances, though mechanisms are mostly unknown. We studied wound-healing processes of human primary fibroblasts, by exposing cells to a platelet lysate (PL) obtained from blood samples. Crystal violet and tetrazolium salt (MTS) assays showed dose-response increase of cell proliferation and metabolism. In scratch wound and transwell assays, a dose of 20% PL induced a significant increase of wound closure rate at 6 and 24 hrs, and had a strong chemotactic effect. BAPTA-AM, SB203580 and PD98059 caused 100% inhibition of PL effects, whereas wortmannin reduced to about one third the effect of PL on wound healing and abolished the chemotactic response. Confocal imaging showed the induction by PL of serial Ca2(+) oscillations in fibroblasts. Data indicate that cell Ca2(+) plays a fundamental role in wound healing even without PL, p38 and ERK1/2 are essential for PL effects but are also activated by wounding per se, PI3K is essential for PL effects and its downstream effector Akt is activated only in the presence of PL. In conclusion, PL stimulates fibroblast wound healing through the activation of cell proliferation and motility with different patterns of involvement of different signalling pathways.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.