ArticleMethods in molecular biology (Clifton, N.J.)2009
Gene targeting in mouse embryonic stem cells.
Article in Methods in molecular biology (Clifton, N.J.), 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
14 citing papers in PubMed, 25 citations in OpenAlex.
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- Abcb5-deficient mice show a subtle, pleiotropic phenotype indicating a role for this transporter in intermediary metabolism.iScience · 2025Article
- Telomerase reverse transcriptase gene knock-in unleashes enhanced longevity and accelerated damage repair in mice.Aging cell · 2025Article
- Transcriptomic analysis reveals that RasGEF1b deletion alters basal and LPS-induced expression of genes involved in chemotaxis and cytokine responses in macrophages.Scientific reports · 2023Article
- Fatty acid oxidation is required for embryonic stem cell survival during metabolic stress.EMBO reports · 2021Article
- Far Upstream Element Binding Protein Plays a Crucial Role in Embryonic Development, Hematopoiesis, and Stabilizing Myc Expression Levels.The American journal of pathology · 2016Article
- Fgf3-Fgf4-cis: A new mouse line for studying Fgf functions during mouse development.Genesis (New York, N.Y. : 2000) · 2016Article
- An NF-κB--EphrinA5-Dependent Communication between NG2(+) Interstitial Cells and Myoblasts Promotes Muscle Growth in Neonates.Developmental cell · 2016Article
- Normal viability of Kai1/Cd82 deficient mice.Molecular carcinogenesis · 2014Article
- Detection of differential fetal and adult expression of chloride intracellular channel 4 (CLIC4) protein by analysis of a green fluorescent protein knock-in mouse line.BMC developmental biology · 2014Article
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- Tamalin is a critical mediator of electroconvulsive shock-induced adult neuroplasticity.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2012Article
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- SSSPTA is essential for serine palmitoyltransferase function during development and hematopoiesis.The Journal of biological chemistryArticle
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
The scientific value of a mouse model with a targeted mutation depends greatly upon how carefully the mutation has been engineered. Until recently, our ability to alter the mouse genome has been limited by both the lack of technologies to conditionally target a locus and by conventional cloning. The "cre/loxP" and "recombineering" technologies have overcome some of these limitations and have greatly enhanced our ability to manipulate the mouse genome in a sophisticated way. However, there are still some practical aspects that need to be considered to successfully target a specific genetic locus. Here, we describe the process to engineer a targeted mutation to generate a mouse model. We include a tutorial using the publicly available informatic tools that can be downloaded for processing the genetic information needed to generate a targeting vector.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.