ArticleNature nanotechnology2009
Quantitative time-resolved measurement of membrane protein-ligand interactions using microcantilever array sensors.
Article in Nature nanotechnology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 40 papers.
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Who cites it
40 citing papers in PubMed, 223 citations in OpenAlex.
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- Rapid and Ultrasensitive Detection of Mutations and Genes Relevant to Antimicrobial Resistance in Bacteria.Global challenges (Hoboken, NJ) · 2021Article
- Nanomotion detection based on atomic force microscopy cantilevers.Cell surface (Amsterdam, Netherlands) · 2019Review
- Biomimetic Membranes with Transmembrane Proteins: State-of-the-Art in Transmembrane Protein Applications.International journal of molecular sciences · 2019Review
- A Rapid Unraveling of the Activity and Antibiotic Susceptibility of Mycobacteria.Antimicrobial agents and chemotherapy · 2019Article
- Experimental Setup for Dynamic Analysis of Micro- and Nano-Mechanical Systems in Vacuum, Gas, and Liquid.Micromachines · 2019Article
- Simultaneous and Ultrasensitive Detection of Foodborne Bacteria by Gold Nanoparticles-Amplified Microcantilever Array Biosensor.Frontiers in chemistry · 2019Article
- Modified cantilever arrays improve sensitivity and reproducibility of nanomechanical sensing in living cells.Communications biology · 2018Article
- Acoustic biosensors.Essays in biochemistry · 2016Review
- Finite Element Analysis on Nanomechanical Detection of Small Particles: Toward Virus Detection.Frontiers in microbiology · 2016Article
- An optimised silicon piezoresistive microcantilever sensor for surface stress studies.Microsystem technologies : sensors, actuators, systems integration · 2016Article
- Toward Higher-Order Mass Detection: Influence of an Adsorbate's Rotational Inertia and Eccentricity on the Resonant Response of a Bernoulli-Euler Cantilever Beam.Sensors (Basel, Switzerland) · 2015Article
- Design of surface modifications for nanoscale sensor applications.Sensors (Basel, Switzerland) · 2015Review
- Detecting nanoscale vibrations as signature of life.Proceedings of the National Academy of Sciences of the United States of America · 2015Article
Corrections and comments
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Authors and funding
10 authors at 4 institutions in 4 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Membrane proteins are central to many biological processes, and the interactions between transmembrane protein receptors and their ligands are of fundamental importance in medical research. However, measuring and characterizing these interactions is challenging. Here we report that sensors based on arrays of resonating microcantilevers can measure such interactions under physiological conditions. A protein receptor--the FhuA receptor of Escherichia coli--is crystallized in liposomes, and the proteoliposomes then immobilized on the chemically activated gold-coated surface of the sensor by ink-jet spotting in a humid environment, thus keeping the receptors functional. Quantitative mass-binding measurements of the bacterial virus T5 at subpicomolar concentrations are performed. These experiments demonstrate the potential of resonating microcantilevers for the specific, label-free and time-resolved detection of membrane protein-ligand interactions in a micro-array format.
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Registered trials
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