ArticleThe Journal of biological chemistry2009
Heat shock protein gp96 interacts with protein phosphatase 5 and controls toll-like receptor 2 (TLR2)-mediated activation of extracellular signal-regulated kinase (ERK) 1/2 in post-hypoxic kidney cells.
Article in The Journal of biological chemistry, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 45 citations in OpenAlex.
- Cell- and Serum-Derived Proteins Act as DAMPs to Activate RAW 264.7 Macrophage-like Cells on Silicone Implants.ACS biomaterials science & engineering · 2024Article
- Epigenetic regulation of Toll-like receptors 2 and 4 in kidney disease.Journal of molecular medicine (Berlin, Germany) · 2022Review
- Structure and function of the co-chaperone protein phosphatase 5 in cancer.Cell stress & chaperones · 2020Review
- Regulatory T Cells Condition Lymphatic Endothelia for Enhanced Transendothelial Migration.Cell reports · 2020Article
- Vascular Signaling in Allogenic Solid Organ Transplantation - The Role of Endothelial Cells.Frontiers in physiology · 2020Review
- Berberine nanoparticles protects tubular epithelial cells from renal ischemia-reperfusion injury.Oncotarget · 2017Article
- Serine/Threonine Protein Phosphatase-5 Accelerates Cell Growth and Migration in Human Glioma.Cellular and molecular neurobiology · 2015Article
- Pattern recognition receptors and the inflammasome in kidney disease.Nature reviews. Nephrology · 2014Review
- Protein phosphatases in pancreatic islets.The Journal of endocrinology · 2014Review
- The modulation of phosphatase expression impacts the proliferation efficiency of HSV-1 in infected astrocytes.PloS one · 2013Article
- Synchronous recruitment of epigenetic modifiers to endotoxin synergistically activated Tnf-α gene in acute kidney injury.PloS one · 2013Article
- Delayed ischemic preconditioning contributes to renal protection by upregulation of miR-21.Kidney international · 2012Article
- ERK2-dependent activation of c-Jun is required for nontypeable Haemophilus influenzae-induced CXCL2 upregulation in inner ear fibrocytes.Journal of immunology (Baltimore, Md. : 1950) · 2012Article
- Disruption of serine/threonine protein phosphatase 5 (PP5:PPP5c) in mice reveals a novel role for PP5 in the regulation of ultraviolet light-induced phosphorylation of serine/threonine protein kinase Chk1 (CHEK1).The Journal of biological chemistry · 2011Article
- Dangers within: DAMP responses to damage and cell death in kidney disease.Journal of the American Society of Nephrology : JASN · 2011Review
- Differential activation of Toll-like receptor-mediated apoptosis induced by hypoxia.Oncotarget · 2010Review
- HMGB1 contributes to kidney ischemia reperfusion injury.Journal of the American Society of Nephrology : JASN · 2010Article
- Toll-like receptor 2 mediates inhibition of HCO(3)(-) absorption by bacterial lipoprotein in medullary thick ascending limb.American journal of physiology. Renal physiology · 2010Article
- The role of Toll-like receptors in renal diseases.Nature reviews. Nephrology · 2010Review
- The protective role of pregnane X receptor in lipopolysaccharide/D-galactosamine-induced acute liver injury.Laboratory investigation; a journal of technical methods and pathology · 2010Article
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Authors and funding
7 authors at 5 institutions in 1 country.
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No grant is acknowledged in the PubMed record.
Abstract
Ischemia/reperfusion injury (IRI) induces an innate immune response, leading to an inflammatory reaction and tissue damage that have been attributed to engagement of the Toll-like receptor (TLR) 2 and 4. However, the respective roles of TLR2 and/or TLR4 in mediating downstream activation of mitogen-activated protein kinase (MAPK) pathways during IRI have not been fully elucidated. Here we show that extracellular signal-regulated kinase (ERK)1/2 is activated in both intact kidneys and cultured renal tubule epithelial cells (RTECs) from wildtype and Tlr4 knockout mice, but not those from Tlr2 knockout mice subjected to transient ischemia. Geldanamycin (GA), an inhibitor of heat shock protein 90 and reticulum endoplasmic-resident gp96, and gp96 mRNA silencing (siRNA), did not affect ERK1/2 activation in either post-hypoxic wild-type or Tlr4-deficient RTECs, but did restore its activation in post-hypoxic Tlr2-deficient RTECs. Immunoprecipitation studies revealed that gp96 co-immunoprecipitates with the serine-threonine protein phosphatase 5 (PP5), identified as a negative modulator of the mitogen extracellular kinase (MEK)-ERK pathway, in unstressed wild-type and post-hypoxic Tlr2-deficient RTECs. In contrast, PP5 co-immunoprecipitation with gp96 was strikingly reduced in post-hypoxic wild-type RTECs, suggesting that the inactivation of PP5 resulting from the dissociation of PP5 from gp96 allows the activation of ERK1/2 to occur. Inhibition of PP5 by okadaic acid, and Pp5 siRNA also restored TLR2-mediated phosphorylation of ERK1/2, and apoptosis signal-regulating kinase 1 (ASK1)/c-Jun N-terminal kinase (JNK)-mediated apoptosis in post-hypoxic Tlr2-deficient RTECs. These findings indicate that gp96 interacts with PP5 and controls TLR2-mediated induction of ERK1/2 in post-hypoxic renal tubule cells.
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