ArticleJournal of experimental botany2009
Biochemical characterization, mitochondrial localization, expression, and potential functions for an Arabidopsis gamma-aminobutyrate transaminase that utilizes both pyruvate and glyoxylate.
Article in Journal of experimental botany, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 48 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
48 citing papers in PubMed, 114 citations in OpenAlex.
- Crystal structure of the plant GABA aminotransferase AtGABA-T from Arabidopsis thaliana.Acta crystallographica. Section F, Structural biology communications · 2026Article
- Mapping multi-substrate specificity of Arabidopsis aminotransferases.Nature plants · 2025Article
- Synthesis of β-Alanine From Isoleucine and Propionate Catabolism via Aminotransferases.Plant direct · 2024Article
- Role of Neurotransmitters (Biomediators) in Plant Responses to Stress.Plants (Basel, Switzerland) · 2024Review
- Relationship between the GABA Pathway and Signaling of Other Regulatory Molecules.International journal of molecular sciences · 2024Review
- Functional characterization ofBiochemistry and biophysics reports · 2024Article
- Review
- GABA Metabolism, Transport and Their Roles and Mechanisms in the Regulation of Abiotic Stress (Hypoxia, Salt, Drought) Resistance in Plants.Metabolites · 2023Review
- Phytochromes and Their Role in Diurnal Variations of ROS Metabolism and Plant Proteome.International journal of molecular sciences · 2022Article
- An Aminobutyric Acid Transaminase inFrontiers in plant science · 2022Article
- γ-Aminobutyrate Improves the Postharvest Marketability of Horticultural Commodities: Advances and Prospects.Frontiers in plant science · 2022Review
- The emerging role of GABA as a transport regulator and physiological signal.Plant physiology · 2021Review
- GABA: A Key Player in Drought Stress Resistance in Plants.International journal of molecular sciences · 2021Review
- γ-Aminobutyrate (GABA) Regulated Plant Defense: Mechanisms and Opportunities.Plants (Basel, Switzerland) · 2021Review
- The Impact of Photorespiratory Glycolate Oxidase Activity onMetabolites · 2021Article
- Cytidine-to-Uridine RNA Editing Factor NbMORF8 Negatively Regulates Plant Immunity toPlant physiology · 2020Article
- Keep Calm and Survive: Adaptation Strategies to Energy Crisis in Fruit Trees under Root Hypoxia.Plants (Basel, Switzerland) · 2020Review
- Rapid Single-Step Affinity Purification of HA-Tagged Plant Mitochondria.Plant physiology · 2020Article
- Alternative Oxidase Isoforms Are Differentially Activated by Tricarboxylic Acid Cycle Intermediates.Plant physiology · 2018Article
- Transcriptomic response to GABA-producing Lactobacillus plantarum CGMCC 1.2437T induced by L-MSG.PloS one · 2018Article
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Gamma-aminobutyrate transaminase (GABA-T) catalyses the breakdown of GABA to succinic semialdehyde. In this report, the previously identified Arabidopsis thaliana (L.) Heyhn GABA-T (AtGABA-T) was characterized in more detail. Full-length AtGABA-T contains an N-terminal 36 amino acid long targeting pre-sequence (36 amino acids) that is both sufficient and necessary for targeting the enzyme to mitochondria. Removal of the pre-sequence encoding this N-terminal targeting domain and co-expression of the resulting truncated AtGABA-T cDNA with the GroES/EL molecular chaperone complex in Escherichia coli yielded good recovery of the soluble recombinant proteins. Activity assays indicated that purified recombinant GABA-T has both pyruvate- and glyoxylate-dependent activities, but cannot utilize 2-oxoglutarate as amino acceptor. Kinetic parameters for glyoxylate- and pyruvate-dependent GABA-T activities were similar, with physiologically relevant affinities. Assays of GABA-T activity in cell-free leaf extracts from wild-type Arabidopsis and two knockout mutants in different genetic backgrounds confirmed that the native enzyme possesses both pyruvate- and glyoxylate-dependent activities. The GABA-T transcript was present throughout the plant, but its expression was highest in roots and increased as a function of leaf development. A GABA-T with dual functions suggests the potential for interaction between GABA metabolism and photorespiratory glyoxylate production.
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