ArticleRNA (New York, N.Y.)2009
E. coli RNase III(E38A) generates discrete-sized products from long dsRNA.
Article in RNA (New York, N.Y.), 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 29 citations in OpenAlex.
- The HigB-like SehA toxin promotes non-replicating Salmonella inside macrophages by inhibiting ribonuclease III-dependent rRNA maturation.Nucleic acids research · 2026Article
- Fitness and Functional Landscapes of the E. coli RNase III Gene rnc.Molecular biology and evolution · 2023Article
- Structural basis for Dicer-like function of an engineered RNase III variant and insights into the reaction trajectory of two-MgRNA biology · 2022Article
- RNase III, Ribosome Biogenesis and Beyond.Microorganisms · 2021Review
- Expression of Human ACE2 N-terminal Domain, Part of the Receptor for SARS-CoV-2, in Fusion With Maltose-Binding Protein,Frontiers in microbiology · 2021Article
- Article
- The molecular mechanism of dsRNA processing by a bacterial Dicer.Nucleic acids research · 2019Article
- Article
- miR302 regulates SNAI1 expression to control mesangial cell plasticity.Scientific reports · 2017Article
- siPools: highly complex but accurately defined siRNA pools eliminate off-target effects.Nucleic acids research · 2014Article
- Functional conservation of RNase III-like enzymes: studies on a Vibrio vulnificus ortholog of Escherichia coli RNase III.Current microbiology · 2014Article
- Two tandem RNase III cleavage sites determine betT mRNA stability in response to osmotic stress in Escherichia coli.PloS one · 2014Article
- Production of highly potent recombinant siRNAs in Escherichia coli.Nature protocols · 2013Article
- Inhibition of ribosomal subunit synthesis in Escherichia coli by the vanadyl ribonucleoside complex.Current microbiology · 2013Article
- RNase III: Genetics and function; structure and mechanism.Annual review of genetics · 2013Review
- Enhanced virus resistance in transgenic maize expressing a dsRNA-specific endoribonuclease gene from E. coli.PloS one · 2013Article
- RNase III controls the degradation of corA mRNA in Escherichia coli.Journal of bacteriology · 2012Article
- Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Ribonuclease III (RNase III) represents a highly conserved family of double-strand-specific endoribonucleases that are important for RNA processing and post-transcriptional gene regulation in both prokaryotes and eukaryotes. We constructed a single amino acid substitution (E38A) of RNase III that shows a unique and useful enzymatic activity. It produces a dsRNA product of a discrete size migrating as 23 base pairs (bp) when given a long dsRNA as a substrate in an easy-to-control reaction. We demonstrate that the RNase III(E38A) mutant produces the 23-bp dsRNA product by making a double-strand cleavage of the long dsRNA substrate with the product being protected from further digestion. Using the hairpin RNA R1.1 as a substrate, RNase III(E38A) cleaves at the primary site and remains bound to the RNA, thereby preventing cleavage at the secondary site. The 23-bp dsRNA product is demonstrated to be a pool of dsRNAs representative of the long dsRNA substrate and has RNA interference activity in mammalian tissue culture transfection experiments. The RNA interference activity suggests that the 23-bp dsRNA product has typical 2-nucleotide 3' overhangs and behaves as siRNA thereby making it a useful tool in RNA interference experiments.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.