ArticleMolecular biology reports2010
RA induces the neural-like cells generated from epigenetic modified NIH/3T3 cells.
Article in Molecular biology reports, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed, 17 citations in OpenAlex.
- Induced neural stem/precursor cells for fundamental studies and potential application in neurodegenerative diseases.Neuroscience bulletin · 2015Review
- Cellular extract facilitates nuclear reprogramming by altering DNA methylation and pluripotency gene expression.Cellular reprogramming · 2014Article
- Cardiomyocyte marker expression in mouse embryonic fibroblasts by cell-free cardiomyocyte extract and epigenetic manipulation.Iranian journal of medical sciences · 2014Article
- Expression of a2, a5 and a6 subunits of integrin in de-differentiated NIH3T3 cells by cell-free extract of embryonic stem cells.Molecular biology reports · 2012Article
- Expression of pluripotency markers in human granulosa cells after embryonic stem cell extract exposure and epigenetic modification.Iranian journal of reproductive medicine · 2012Article
- Methylated promoters of genes encoding protocadherins as a new cancer biomarker family.Molecular biology reports · 2012Article
- Article
- Down-regulation of achaete-scute complex homolog 1 (ASCL1) in neuroblastoma cells induces up-regulation of insulin-like growth factor 2 (IGF2).Molecular biology reports · 2011Article
- Cardiomyocyte marker expression in a human lymphocyte cell line using mouse cardiomyocyte extract.Human cell · 2011Article
- Epigenetic modifiers are necessary but not sufficient for reprogramming non-myelinating cells into myelin gene-expressing cells.PloS one · 2010Article
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Recently, differentiated somatic cells had been reprogrammed to pluripotential state in vitro, and various tissue cells had been elicited from those cells. Epigenetic modifications allow differentiated cells to perpetuate the molecular memory needed for the cells to retain their identity. DNA methylation and histone deacetylation are important patterns involved in epigenetic modification, which take critical roles in regulating DNA expression. In this study, we dedifferentiated NIH/3T3 fibroblasts by 5-aza-2-deoxycytidine (5-aza-dC) and Trichstatin A (TSA) combination, and detected gene expression pattern, DNA methylation level, and differentiation potential of reprogrammed cells. As the results, embryonic marker Sox2, klf4, c-Myc and Oct4 were expressed in reprogrammed NIH/3T3 fibroblasts. Total DNA methylation level was significant decreased after the treatment. Moreover, exposure of the reprogrammed cells to all trans-retinoic acid (RA) medium elicited the generation of neuronal class IIIbeta-tubulin-positive, neuron-specific enolase (NSE)-positive, nestin-positive, and neurofilament light chain (NF-L)-positive neural-like cells.
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