Evidence map›Paper›PMID 19263076›Full record

ArticlePlanta2009

Controlled indole-3-acetaldoxime production through ethanol-induced expression of CYP79B2.

Michael Dalgaard Mikkelsen, Victoria L Fuller, Bjarne Gram Hansen, Majse Nafisi, Carl Erik Olsen, Henrik Bjørn Nielsen, Barbara Ann Halkier

Abstract read
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In one paragraph

Article in Planta, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.

0numbers the graph read from it
0cells of the map it votes in
8citing papers in PubMed
0.7field-weighted citation impact, top 31% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

8 citing papers in PubMed, 16 citations in OpenAlex.

  1. Article
  2. Article
  3. Article
  4. Transcriptome analysis of leaf senescence in red clover (Physiology and molecular biology of plants : an international journal of functional plant biology · 2018
    Article
  5. Review
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 2 institutions in 1 country.

Michael Dalgaard MikkelsenVKR Research Centre Pro-Active Plants, Department of Plant Biology and Biotechnology, University of Copenhagen, 40 Thorvaldsensvej, 1871, Frederiksberg C, Denmark.
Victoria L Fuller
Bjarne Gram Hansen
Majse Nafisi
Carl Erik Olsen
Henrik Bjørn Nielsen
Barbara Ann Halkier
University of Copenhagen · DKTechnical University of Denmark · DK

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Indole-3-acetaldoxime (IAOx) is a key branching point between primary and secondary metabolism. IAOx serves as an intermediate in the biosynthesis of indole glucosinolates (I-GLSs), camalexin and the plant hormone indole-3-acetic acid (IAA). The cytochrome P450s CYP79B2 and CYP79B3 catalyze the conversion of tryptophan to IAOx. CYP83B1 channels IAOx into I-GLS biosynthesis, CYP71A13 channels IAOx into camalexin biosynthesis, whereas the IAOx-metabolizing enzyme in IAA biosynthesis is not known. In this report, we demonstrate controlled production of I-GLSs by introducing an ethanol (EtOH)-inducible CYP79B2 construct into double (cyp79b2 cyp79b3) or triple (cyp79b2 cyp79b3 cyp83b1) mutant lines. We show EtOH-dependent induction of camalexin and identify a number of candidate IAA homeostasis- or defense-related genes by clustered microarray analysis. The transgenic mutant lines are thus promising tools for elucidating the interplay between primary and secondary metabolism.

Indexed as

ArabidopsisArabidopsis ProteinsBiosynthetic PathwaysChromatography, LiquidCluster AnalysisCytochrome P-450 Enzyme SystemEthanolGene Expression ProfilingGene Expression Regulation, EnzymologicGene Expression Regulation, PlantGlucosinolatesIndoleacetic AcidsIndolesMass SpectrometryMolecular StructureMutationArabidopsis ProteinscamalexinCYP83B1 protein, Arabidopsiscytochrome P-450 79B2 protein, Arabidopsiscytochrome P-450 CYP79B3, Sinapis albaCytochrome P-450 Enzyme SystemEthanolGlucosinolatesindole-3-acetaldoximeindoleacetic acidIndoleacetic AcidsIndolesOximesPhytoalexinsThiazoles

Identifiers

PMID19263076
OpenAlexW2012740121

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.