ArticleCell adhesion & migration
Actin-cytoskeleton dynamics in non-monotonic cell spreading.
Article in Cell adhesion & migration. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 29 citations in OpenAlex.
- Coordinated Arp2/3-mediated actin branching and myosin-dependent force generation in membrane-cytoskeleton remodeling.Frontiers in physiology · 2026Review
- Adhesion tunes speed and persistence by coordinating protrusions and extracellular matrix remodeling.Developmental cell · 2023Article
- Cells responding to chemoattractant on a structured substrate.Biophysical journal · 2022Article
- Quantifying protrusions as tumor-specific biophysical predictors of cancer invasion in in vitro tumor micro-spheroid models.In vitro models · 2022Article
- The influence of cell elastic modulus on inertial positions in Poiseuille microflows.Biophysical journal · 2021Article
- Formins specify membrane patterns generated by propagating actin waves.Molecular biology of the cell · 2020Article
- Actin Waves and Dynamic Patterning of the Plasma Membrane.The Yale journal of biology and medicine · 2019Article
- From Phagocytes to Immune Defense: Roles for Coronin Proteins inFrontiers in cellular and infection microbiology · 2018Review
- Real-time monitoring of cell protrusion dynamics by impedance responses.Scientific reports · 2015Article
- Moving towards a paradigm: common mechanisms of chemotactic signaling in Dictyostelium and mammalian leukocytes.Cellular and molecular life sciences : CMLS · 2014Review
- Reversible membrane pearling in live cells upon destruction of the actin cortex.Biophysical journal · 2014Article
- Dynamic localization of the actin-bundling protein cortexillin I during cell migration.Molecules and cells · 2011Article
- Spreading dynamics of biomimetic actin cortices.Biophysical journal · 2011Article
- A high precision survey of the molecular dynamics of mammalian clathrin-mediated endocytosis.PLoS biology · 2011Article
- Regulation of Hip1r by epsin controls the temporal and spatial coupling of actin filaments to clathrin-coated pits.Journal of cell science · 2010Article
- Self-organizing actin waves that simulate phagocytic cup structures.PMC biophysics · 2010Article
- Propagating waves separate two states of actin organization in living cells.HFSP journal · 2009Article
- The three-dimensional dynamics of actin waves, a model of cytoskeletal self-organization.Biophysical journal · 2009Article
Corrections and comments
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Authors and funding
8 authors at 6 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The spreading of motile cells on a substrate surface is accompanied by reorganization of their actin network. We show that spreading in the highly motile cells of Dictyostelium is non-monotonic, and thus differs from the passage of spreading cells through a regular series of stages. Quantification of the gain and loss of contact area revealed fluctuating forces of protrusion and retraction that dominate the interaction of Dictyostelium cells with a substrate. The molecular basis of these fluctuations is elucidated by dual-fluorescence labeling of filamentous actin together with proteins that highlight specific activities in the actin system. Front-to-tail polarity is established by the sorting out of myosin-II from regions where dense actin assemblies are accumulating. Myosin-IB identifies protruding front regions, and the Arp2/3 complex localizes to lamellipodia protruded from the fronts. Coronin is used as a sensitive indicator of actin disassembly to visualize the delicate balance of polymerization and depolymerization in spreading cells. Short-lived actin patches that co-localize with clathrin suggest that membrane internalization occurs even when the substrate-attached cell surface expands. We conclude that non-monotonic cell spreading is characterized by spatiotemporal patterns formed by motor proteins together with regulatory proteins that either promote or terminate actin polymerization on the scale of seconds.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.