ArticleJournal of experimental botany2009
Functional analysis of the isoforms of an ABI3-like factor of Pisum sativum generated by alternative splicing.
Article in Journal of experimental botany, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
16 citing papers in PubMed, 24 citations in OpenAlex.
- Regulatory Effects of ABA and GA on the Expression of Conglutin Genes andInternational journal of molecular sciences · 2023Article
- Alternative splicing in ABA signaling during seed germination.Frontiers in plant science · 2023Review
- Understanding of Hormonal Regulation in Rice Seed Germination.Life (Basel, Switzerland) · 2022Review
- Article
- Post-transcriptional regulation of seed dormancy and germination: Current understanding and future directions.Plant communications · 2021Review
- Aethionema arabicum genome annotation using PacBio full-length transcripts provides a valuable resource for seed dormancy and Brassicaceae evolution research.The Plant journal : for cell and molecular biology · 2021Article
- Transcriptome Analysis of Alternative Splicing Events Induced by Arbuscular Mycorrhizal Fungi (Plants (Basel, Switzerland) · 2020Article
- Vegetative desiccation tolerance in the resurrection plant Xerophyta humilis has not evolved through reactivation of the seed canonical LAFL regulatory network.The Plant journal : for cell and molecular biology · 2020Article
- Molecular and epigenetic regulations and functions of the LAFL transcriptional regulators that control seed development.Plant reproduction · 2018Review
- Molecular Mechanisms Underlying Abscisic Acid/Gibberellin Balance in the Control of Seed Dormancy and Germination in Cereals.Frontiers in plant science · 2018Review
- Article
- Alternative Splicing as a Regulator of Early Plant Development.Frontiers in plant science · 2018Review
- Complexity of the alternative splicing landscape in plants.The Plant cell · 2013Review
- Functional characterization of two alternatively spliced transcripts of tomato ABSCISIC ACID INSENSITIVE3 (ABI3) gene.Plant molecular biology · 2013Article
- The conserved splicing factor SUA controls alternative splicing of the developmental regulator ABI3 in Arabidopsis.The Plant cell · 2010Article
- Cross-species approaches to seed dormancy and germination: conservation and biodiversity of ABA-regulated mechanisms and the Brassicaceae DOG1 genes.Plant molecular biology · 2010Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
At least seven isoforms (PsABI3-1 to PsABI3-7) of a putative, pea ABI3-like factor, originated by alternative splicing, have been identified after cDNA cloning. A similar variability had previously only been described for monocot genes. The full-length isoform, PsABI3-1, contains the typical N-terminal acidic domains and C-terminal basic subdomains, B1 to B3. Reverse transcriptase-PCR analysis revealed that the gene is expressed just in seeds, starting at middle embryogenesis; no gene products are observed in embryo axes after 18 h post-imbibition although they are more persistent in cotyledons. The activity of the isoforms was studied by yeast one-hybrid assays. When yeast was transformed with the isoforms fused to the DNA binding domain of Gal4p, only the polypeptides PsABI3-2 and PsABI3-7 failed to complement the activity of Gal4p. Acidic domains A1 and A2 exhibit transactivating activity, but the former requires a small C-terminal extension to be active. Yeast two-hybrid analysis showed that PsABI3 is able to heterodimerize with Arabidopsis thaliana ABI5, thus proving that PsABI3 is functionally active. The minimum requirement for the interaction PsABI3-AtABI5 is the presence of the subdomain B1 with an extension, 81 amino acids long, at their C-terminal side. Finally, a transient onion transformation assay showed that both the active PsABI3-1 and the inactive PsABI3-2 isoforms are localized to nuclei. Considering that the major isoforms remain approximately constant in developing seeds although their relative proportion varied, the possible role of splicing in the regulatory network of ABA signalling is discussed.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.