ArticleThe Journal of neuroscience : the official journal of the Society for Neuroscience2009
Role of calcineurin signaling in membrane potential-regulated maturation of cerebellar granule cells.
Article in The Journal of neuroscience : the official journal of the Society for Neuroscience, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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24 citing papers in PubMed, 36 citations in OpenAlex.
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- Developmentally Transient CB1Rs on Cerebellar Afferents Suppress Afferent Input, Downstream Synaptic Excitation, and Signaling to Migrating Neurons.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2020Article
- Developmental pattern and structural factors of dendritic survival in cerebellar granule cells in vivo.Scientific reports · 2018Article
- Neuritin promotes neurite and spine growth in rat cerebellar granule cells via L-type calcium channel-mediated calcium influx.Journal of neurochemistry · 2018Article
- Consensus Paper: Cerebellar Development.Cerebellum (London, England) · 2016Review
- Organotypic Spinal Cord Culture: a Proper Platform for the Functional Screening.Molecular neurobiology · 2016Review
- Evaluating cell reprogramming, differentiation and conversion technologies in neuroscience.Nature reviews. Neuroscience · 2016Review
- RCAN1 overexpression promotes age-dependent mitochondrial dysregulation related to neurodegeneration in Alzheimer's disease.Acta neuropathologica · 2015Article
- Maturation of membrane properties of neurons in the rat deep cerebellar nuclei.Developmental neurobiology · 2014Article
- Cell-intrinsic drivers of dendrite morphogenesis.Development (Cambridge, England) · 2013Review
- The type II cGMP dependent protein kinase regulates GluA1 levels at the plasma membrane of developing cerebellar granule cells.Biochimica et biophysica acta · 2013Article
- Temporal regulation of nuclear factor one occupancy by calcineurin/NFAT governs a voltage-sensitive developmental switch in late maturing neurons.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2013Article
- Gene regulation via excitation and BDNF is mediated by induction and phosphorylation of the Etv1 transcription factor in cerebellar granule cells.Proceedings of the National Academy of Sciences of the United States of America · 2012Article
- Nurr1 protein is required for N-methyl-D-aspartic acid (NMDA) receptor-mediated neuronal survival.The Journal of biological chemistry · 2012Article
- Calcineurin serves in the circadian output pathway to regulate the daily rhythm of L-type voltage-gated calcium channels in the retina.Journal of cellular biochemistry · 2012Article
- Models of calcium dynamics in cerebellar granule cells.Cerebellum (London, England) · 2012Review
- Subcellular TSC22D4 localization in cerebellum granule neurons of the mouse depends on development and differentiation.Cerebellum (London, England) · 2012Article
- Transient expression of functional serotonin 5-HT3 receptors by glutamatergic granule cells in the early postnatal mouse cerebellum.The Journal of physiology · 2011Article
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
At the early postnatal period, cerebellar granule cells proliferate, differentiate, migrate, and finally form refined synaptic connections with mossy fibers. During this period, the resting membrane potential of immature granule cells is relatively depolarized, but it becomes hyperpolarized in mature cells. This investigation was conducted to examine the role of this alteration in membrane potential and its downstream signaling mechanism in development and maturation of granule cells. Experiments were designed to precisely characterize the ontogenic processes of developing granule cells by combining organotypic cerebellar cultures with the specific expression of EGFP (enhanced green fluorescent protein) in granule cells by use of DNA transfection. Multiple approaches using morphology, electrophysiology, and immunohistochemistry demonstrated that granule cells developed and matured at the physiological KCl concentration in organotypic cultures in a temporally regulated manner. We addressed how persistent membrane depolarization influences the developmental and maturation processes of granule cells by depolarizing organotypic cultures with high KCl. Depolarization preserved the developmental processes of granule cells up to the stage of formation of immature dendrites but prevented the maturation processes for synaptic formation by granule cells. Importantly, this blockade of the terminal maturation of granule cells was reversed by inactivation of calcineurin with its specific inhibitor. This investigation has demonstrated that alteration of the membrane potential and its downstream calcineurin signaling play a pivotal role in triggering the maturation program for the synaptic organization of postnatally developing granule cells.
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