ArticleProceedings of the National Academy of Sciences of the United States of America2009
Demonstration of the ethylmalonyl-CoA pathway by using 13C metabolomics.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 113 papers.
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Who cites it
113 citing papers in PubMed, 234 citations in OpenAlex.
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- Integrated genomic and transcriptomic Insights into methanol tolerance mechanisms in Methylobacterium extorquens AM1, identifying key targets for strain engineering.Journal of biological engineering · 2025Article
- XoxF and the Calvin-Benson cycle mediate lanthanide-dependent growth on methanol inApplied and environmental microbiology · 2025Article
- Pregnenolone Bioproduction in Engineered Methylobacteria: Design and Elaboration.International journal of molecular sciences · 2025Article
- New-to-nature CONature communications · 2024Article
- Transcriptomic data reveals an auxiliary detoxification mechanism that alleviates formaldehyde stress in Methylobacterium sp. XJLW.BMC genomics · 2024Article
- The lactonase BxdA mediates metabolic specialisation of maize root bacteria to benzoxazinoids.Nature communications · 2024Article
- Simultaneous Formate and Syngas Conversion Boosts Growth and Product Formation byMolecules (Basel, Switzerland) · 2024Article
- Constructing efficient bacterial cell factories to enable one-carbon utilization based on quantitative biology: A review.Quantitative biology (Beijing, China) · 2024Review
- With a pinch of salt: metagenomic insights into Namib Desert salt pan microbial mats and halites reveal functionally adapted and competitive communities.Applied and environmental microbiology · 2023Article
- Expression of toxic genes in Methylorubrum extorquens with a tightly repressed, cumate-inducible promoter.Antonie van Leeuwenhoek · 2023Article
- Engineering of thioesterase YciA from Haemophilus influenzae for production of carboxylic acids.Applied microbiology and biotechnology · 2023Article
- The Methylcitrate Cycle and Its Crosstalk with the Glyoxylate Cycle and Tricarboxylic Acid Cycle in Pathogenic Fungi.Molecules (Basel, Switzerland) · 2023Review
- Methanol-based biomanufacturing of fuels and chemicals using native and synthetic methylotrophs.Synthetic and systems biotechnology · 2023Article
- Metabolic resource overlap impacts competition among phyllosphere bacteria.The ISME journal · 2023Article
- Functional Degeneracy in Paracoccus denitrificans Pd1222 Is Coordinated via RamB, Which Links Expression of the Glyoxylate Cycle to Activity of the Ethylmalonyl-CoA Pathway.Applied and environmental microbiology · 2023Article
- An optimized reverse β-oxidation pathway to produce selected medium-chain fatty acids in Saccharomyces cerevisiae.Biotechnology for biofuels and bioproducts · 2023Article
- Comparative Genomic Analysis of aInternational journal of molecular sciences · 2023Article
- Methylotrophic bacteria with cobalamin-dependent mutases in primary metabolism as potential strains for vitamin BAntonie van Leeuwenhoek · 2023Article
53 more citing papers are in PubMed but not listed here.
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Authors and funding
6 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The assimilation of one-carbon (C1) compounds, such as methanol, by serine cycle methylotrophs requires the continuous regeneration of glyoxylate. Instead of the glyoxylate cycle, this process is achieved by a not yet established pathway where CoA thioesters are known to play a key role. We applied state-of-the-art metabolomics and (13)C metabolomics strategies to demonstrate how glyoxylate is generated during methylotrophic growth in the isocitrate lyase-negative methylotroph Methylobacterium extorquens AM1. High-resolution mass spectrometry showed the presence of CoA thioesters specific to the recently proposed ethylmalonyl-CoA pathway. The operation of this pathway was demonstrated by short-term (13)C-labeling experiments, which allowed determination of the sequence of reactions from the order of label incorporation into the different CoA derivatives. Analysis of (13)C positional enrichment in glycine by NMR was consistent with the predicted labeling pattern as a result of the operation of the ethylmalonyl-CoA pathway and the unique operation of the latter for glyoxylate generation during growth on methanol. The results also revealed that 2 molecules of glyoxylate were regenerated in this process. This work provides a complete pathway for methanol assimilation in the model methylotroph M. extorquens AM1 and represents an important step toward the determination of the overall topology of its metabolic network. The operation of the ethylmalonyl-CoA pathway in M. extorquens AM1 has major implications for the physiology of these methylotrophs and their role in nature, and it also provides a common ground for C1 and C2 compound assimilation in isocitrate lyase-negative bacteria.
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