ArticleJournal of clinical microbiology2009
Strain-dependent variation in 18S ribosomal DNA Copy numbers in Aspergillus fumigatus.
Article in Journal of clinical microbiology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 81 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
81 citing papers in PubMed.
- Sources and traits of bacteria and fungi found in the near-surface atmosphere.Applied and environmental microbiology · 2026Article
- Biomarkers in the Diagnosis of Aspergillosis.Journal of fungi (Basel, Switzerland) · 2026Review
- Development of a multiplex ddPCR assay for simultaneous absolute quantification of bacterial, fungal, and human DNA.PloS one · 2026Article
- The Role ofInternational journal of molecular sciences · 2025Article
- Longitudinal dynamics and cross-domain interactions of eukaryotic populations in wastewater treatment plants.The ISME journal · 2025Article
- Predicting how varying moisture conditions impact the microbiome of dust collected from the International Space Station.Microbiome · 2024Article
- Time-series metagenomics reveals changing protistan ecology of a temperate dimictic lake.Microbiome · 2024Article
- Fungal diversity differences in the indoor dust microbiome from built environments on earth and in space.Scientific reports · 2024Article
- A microbial risk assessor's guide to Valley Fever (Coccidioides spp.): Case study and review of risk factors.The Science of the total environment · 2024Review
- Article
- Shotgun metagenomics reveals interkingdom association between intestinal bacteria and fungi involving competition for nutrients.Microbiome · 2023Article
- Simultaneous Analysis of Bacterial and Fungal Communities in Oral Samples from Intubated Patients in Intensive Care Unit.Diagnostics (Basel, Switzerland) · 2023Article
- Species-specific effects of the introduction of Aspergillus nidulans gfdB in osmophilic aspergilli.Applied microbiology and biotechnology · 2023Article
- Porcine fungal mock community analyses: Implications for mycobiome investigations.Frontiers in cellular and infection microbiology · 2023Article
- Quantitative PCR Effectively Quantifies Triazole-Susceptible and Triazole-ResistantJournal of fungi (Basel, Switzerland) · 2022Article
- The role of the Aspergillus nidulans high mobility group B protein HmbA, the orthologue of Saccharomyces cerevisiae Nhp6p.Scientific reports · 2022Article
- Article
- Microbiological and Molecular Diagnosis of Mucormycosis: From Old to New.Microorganisms · 2021Review
- Review
- Comparison of DNA sequencing and morphological identification techniques to characterize environmental fungal communities.Scientific reports · 2021Article
21 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors.
Funding
Abstract
Enumerating Aspergillus fumigatus CFU can be challenging since CFU determination by plate count can be difficult. CFU determination by quantitative real-time PCR (qPCR), however, is becoming increasingly common and usually relies on detecting one of the subunits of the multicopy rRNA genes. This study was undertaken to determine if ribosomal DNA (rDNA) copy number was constant or variable among different A. fumigatus isolates. FKS1 was used as a single-copy control gene and was validated against single-copy (pyrG and ARG4) and multicopy (arsC) controls. The copy numbers of the 18S rDNA subunit were then determined for a variety of isolates and were found to vary with the strain, from 38 to 91 copies per genome. Investigation of the stability of the 18S rDNA copy number after exposure to a number of different environmental and growth conditions revealed that the copy number was stable, varying less than one copy across all conditions, including in isolates recovered from an animal model. These results suggest that while the ribosomal genes are excellent targets for enumeration by qPCR, the copy number should be determined prior to using them as targets for quantitative analysis.
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Registered trials
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