ArticleClinical and vaccine immunology : CVI2009
Antibody response to porcine reproductive and respiratory syndrome virus (PRRSV) nonstructural proteins and implications for diagnostic detection and differentiation of PRRSV types I and II.
Article in Clinical and vaccine immunology : CVI, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 55 papers.
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Who cites it
55 citing papers in PubMed.
- Research progress on NSP4 protein of porcine reproductive and respiratory syndrome virus.Veterinary research communications · 2026Review
- Genetic diversity and pathogenicity of novel recombinant strains of PRRSV lineages 1.5, lineages 1.8 and lineages 8 in South China.Frontiers in cellular and infection microbiology · 2026Article
- Linear epitopes of PRRSV-1 envelope proteins ectodomains are not correlated with broad neutralization.Porcine health management · 2024Article
- Pathogenic Characteristics of Five Different Lineage of Korean PRRSV-2 Isolates (NADC30-Like, VR2332-Like, LKA, LKB, and LKC).Transboundary and emerging diseases · 2024Article
- Research Progress of Porcine Reproductive and Respiratory Syndrome Virus NSP2 Protein.Viruses · 2023Review
- Development of monoclonal antibody-based blocking ELISA for detecting SARS-CoV-2 exposure in animals.mSphere · 2023Article
- Research Progress on Porcine Reproductive and Respiratory Syndrome Virus NSP7 Protein.Animals : an open access journal from MDPI · 2023Review
- Research Progress on NSP11 of Porcine Reproductive and Respiratory Syndrome Virus.Veterinary sciences · 2023Review
- The Omicron Variant BA.1.1 Presents a Lower Pathogenicity than B.1 D614G and Delta Variants in a Feline Model of SARS-CoV-2 Infection.Journal of virology · 2022Article
- The Omicron variant BA.1.1 presents a lower pathogenicity than B.1 D614G and Delta variants in a feline model of SARS-CoV-2 infection.bioRxiv : the preprint server for biology · 2022Article
- Immunogenic and antigenic analysis of recombinant NSP1 and NSP11 of PRRS virus.Veterinary medicine and science · 2022Article
- Article
- Development of a Blocking Enzyme-Linked Immunosorbent Assay for Detection of Antibodies against African Swine Fever Virus.Pathogens (Basel, Switzerland) · 2021Article
- Modernization of Control of Pathogenic Micro-Organisms in the Food-Chain Requires a Durable Role for Immunoaffinity-Based Detection Methodology-A Review.Foods (Basel, Switzerland) · 2021Review
- Isolation of Porcine Reproductive and Respiratory Syndrome Virus GP5-Specific, Neutralizing Monoclonal Antibodies From Hyperimmune Sows.Frontiers in immunology · 2021Article
- Porcine Reproductive and Respiratory Syndrome Virus Promotes SLA-DR-Mediated Antigen Presentation of Nonstructural Proteins To Evoke a Nonneutralizing Antibody ResponseJournal of virology · 2020Article
- Evaluation of Antibody Response Directed against Porcine Reproductive and Respiratory Syndrome Virus Structural Proteins.Vaccines · 2020Article
- A rapid and quantitative fluorescent microsphere immunochromatographic strip test for detection of antibodies to porcine reproductive and respiratory syndrome virus.Journal of veterinary science · 2020Article
- Article
- The PRRSV-Specific Memory B Cell Response Is Long-Lived in Blood and Is Boosted During Live Virus Re-exposure.Frontiers in immunology · 2020Article
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Authors and funding
11 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
To further characterize the humoral immune response of pigs to porcine reproductive and respiratory syndrome virus (PRRSV), direct enzyme-linked immunosorbent assays (ELISA) were used to study the kinetics of antibody responses directed against PRRSV nonstructural proteins in pigs experimentally exposed to the virus. The highest immunoreactivities were against nsp1, nsp2, and nsp7. Using the recombinant nsp7 as an antigen, we validated a dual ELISA for the simultaneous detection and differentiation of serum antibodies against type I and type II PRRSV. Receiver operating characteristic analysis based on 1,334 known-positive and 1,357 known-negative samples showed good specificity (98.3% to type I and 99.3% to type II) and sensitivity (97.4% for type I and 99.8% for type II). To differentiate type I and type II PRRSV, 470 sera originating from experimentally inoculated pigs were tested, and positive sera were correctly differentiated in 469 of 470 samples. The capability of the nsp7 dual ELISA to detect serum antibody responses from pigs infected with various genetically different field strains was determined. The nsp7 dual ELISA possessed 97.6% agreement with the Idexx HerdChek PRRS 2XR ELISA. In further testing of Idexx ELISA suspected false-positive samples, the nsp7 dual ELISA resolved 98% of the samples as negative. Taken together, these results indicate that the nsp7 dual ELISA can be used as a differential test for PRRSV serology with high levels of sensitivity and specificity. This ELISA offers an additional tool for routine or follow-up diagnostics, as well as having substantial value in epidemiological surveys and outbreak investigations.
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