ArticleVeterinary pathology2009
Alcian Blue and Pyronine Y histochemical stains permit assessment of multiple parameters in pulmonary disease models.
Article in Veterinary pathology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
7 citing papers in PubMed, 15 citations in OpenAlex.
- Zinc Deficiency Disturbs Mucin Expression,International journal of molecular sciences · 2020Article
- Intermittent Hypoxic Conditioning Alleviates Post-Traumatic Stress Disorder-Induced Damage and Dysfunction of Rat Visceral Organs and Brain.International journal of molecular sciences · 2020Article
- Glycogen depletion can increase the specificity of mucin detection in airway tissues.BMC research notes · 2018Article
- Immunohistochemical detection of arginase-I expression in formalin-fixed lung and other tissues.Journal of histotechnology · 2013Article
- Genetically modified species in research: Opportunities and challenges for the histology core laboratory.Journal of histotechnology · 2012Article
- Loss of cystic fibrosis transmembrane conductance regulator function produces abnormalities in tracheal development in neonatal pigs and young children.American journal of respiratory and critical care medicine · 2010Article
- Pathology of gastrointestinal organs in a porcine model of cystic fibrosis.The American journal of pathology · 2010Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Utilization of a combined Alcian Blue and Pyronine Y histochemical method for the assessment of multiple parameters in the respiratory tract of various species is described. Acidic mucins were deep blue (sialylated mucins), red (sulfated mucins), or variably purple (mixture of sialylated/sulfated mucins), and differential mucus production was readily detected in a murine respiratory syncytial virus vaccine model of pulmonary inflammation. Elastic fibers stained red in the walls of pulmonary arteries, connecting airways, alveolar septa, and subpleural interstitium. Mast cells had red to red-purple granular cytoplasmic staining. Nuclei were ubiquitously counterstained pale blue. Representative staining was detected in tissues from multiple species, including inbred mice, rats, ferrets, cats, dogs, sheep, and pigs. The fluorescent property of the stained tissues offers additional modalities with which to analyze tissue sections. This histochemical technique detects multiple critical parameters in routine paraffin sections of lung tissue, reduces the need for repeated serial sectioning and staining, and is cost-effective and simple to perform.
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Registered trials
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