ReviewCurrent opinion in chemical biology2009
Advances in generating functional diversity for directed protein evolution.
Review in Current opinion in chemical biology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 41 papers, 1 of them a synthesis that pooled it.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
41 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Preclinical Anticipation of On- and Off-Target Resistance Mechanisms to Anti-Cancer Drugs: A Systematic Review.International journal of molecular sciences · 2024Pooled it
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- Metabolic growth-coupling strategies forMetabolic engineering communications · 2025Article
- Enhanced stereodivergent evolution of carboxylesterase for efficient kinetic resolution of near-symmetric esters through machine learning.Nature communications · 2024Article
- Exploring binding positions and backbone conformations of peptide ligands of proteins with a backbone-centred statistical energy function.Journal of computer-aided molecular design · 2023Article
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- A novel framework for engineering protein loops exploring length and compositional variation.Scientific reports · 2021Article
- Synthesis of fully protected trinucleotide building blocks on a disulphide-linked soluble support.RSC advances · 2021Article
- Synthesis of libraries and multi-site mutagenesis using a PCR-derived, dU-containing template.Synthetic biology (Oxford, England) · 2021Article
- Engineering and application of a biosensor with focused ligand specificity.Nature communications · 2020Article
- Engineering Robust Cellulases for Tailored Lignocellulosic Degradation Cocktails.International journal of molecular sciences · 2020Review
- FeedER: a feedback-regulated enzyme-based slow-release system for fed-batch cultivation in microtiter plates.Bioprocess and biosystems engineering · 2019Article
- Enzyme engineering: A synthetic biology approach for more effective library generation and automated high-throughput screening.PloS one · 2017Article
- In vitro flow cytometry-based screening platform for cellulase engineering.Scientific reports · 2016Article
- Integrated (Meta) Genomic and Synthetic Biology Approaches to Develop New Biocatalysts.Marine drugs · 2016Review
- Article
- Advances in the directed evolution of proteins.Current opinion in chemical biology · 2014Review
- Mutagenic Organized Recombination Process by Homologous IN vivo Grouping (MORPHING) for directed enzyme evolution.PloS one · 2014Article
- Increasing protein production by directed vector backbone evolution.AMB Express · 2013Article
- Expression and function of a modified AP2/ERF transcription factor from Brassica napus enhances cold tolerance in transgenic Arabidopsis.Molecular biotechnology · 2013Article
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5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Despite advances in screening technologies, only a very small fraction of theoretical protein sequence can be sampled in directed evolution experiments. At the current state of random mutagenesis technologies mutation frequencies have often been adjusted to values that cause a limited number of amino acid changes (often one to four amino acid changes per protein). For harvesting the power of directed evolution algorithms it is therefore important that generated mutant libraries are rich in diversity and enriched in active population. Insufficient knowledge about protein traits, mutational robustness of protein folds and technological limitations in diversity generating methods are main challenges for managing the complexity of protein sequence space. This review covers computational and experimental advances for high quality mutant library generation that have been achieved in the past two years.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.