ArticleLangmuir : the ACS journal of surfaces and colloids2009
Measuring diffusion of lipid-like probes in artificial and natural membranes by raster image correlation spectroscopy (RICS): use of a commercial laser-scanning microscope with analog detection.
Article in Langmuir : the ACS journal of surfaces and colloids, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 68 citations in OpenAlex.
- Lipid Rafts in Signalling, Diseases, and Infections: What Can Be Learned from Fluorescence Techniques?Membranes · 2025Review
- CHARMM-GUI Multicomponent Assembler for modeling and simulation of complex multicomponent systems.Nature communications · 2024Article
- Precision Localization of Lipid-Based Nanoparticles by Dual-Fluorescent Labeling for Accurate and High-Resolution Imaging in Living Cells.Small science · 2023Article
- Visualization of subdiffusive sites in a live single cell.Journal of biological methods · 2021Article
- Lateral diffusion of CD14 and TLR2 in macrophage plasma membrane assessed by raster image correlation spectroscopy and single particle tracking.Scientific reports · 2020Article
- Location, Orientation and Aggregation of Bardoxolone-ME, CDDO-ME, in a Complex Phospholipid Bilayer Membrane.The Journal of membrane biology · 2020Article
- Raster Image Correlation Spectroscopy Performance Evaluation.Biophysical journal · 2019Article
- Generation of transgenic zebrafish with 2 populations of RFP- and GFP-labeled thrombocytes: analysis of their lipids.Blood advances · 2019Article
- Arbitrary-Region Raster Image Correlation Spectroscopy.Biophysical journal · 2016Article
- Oligodendroglial membrane dynamics in relation to myelin biogenesis.Cellular and molecular life sciences : CMLS · 2016Review
- Universal Approach to FRAP Analysis of Arbitrary Bleaching Patterns.Scientific reports · 2015Article
- Review
- Modification of plasma membrane organization in tobacco cells elicited by cryptogein.Plant physiology · 2014Article
- Pulsed interleaved excitation fluctuation imaging.Biophysical journal · 2013Article
- Cellular response to heat shock studied by multiconfocal fluorescence correlation spectroscopy.Biophysical journal · 2012Article
- Theory and practical recommendations for autocorrelation-based image correlation spectroscopy.Journal of biomedical optics · 2012Article
- Fluorescence correlation spectroscopy, raster image correlation spectroscopy, and number and brightness on a commercial confocal laser scanning microscope with analog detectors (Nikon C1).Microscopy research and technique · 2011Article
- Quantifying translational mobility in neurons: comparison between current optical techniques.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2010Review
- Raster image correlation spectroscopy in live cells.Nature protocols · 2010Article
- Non-invasive in vivo measurement of the tear film using spatial autocorrelation in a live mammal model.Biomedical optics express · 2010Article
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Authors and funding
10 authors at 4 institutions in 2 countries.
Funding
Abstract
The heterogeneity in composition and interaction within the cellular membrane translates into a wide range of diffusion coefficients of its constituents. Therefore, several complementary microfluorimetric techniques such as fluorescence correlation spectroscopy (FCS), fluorescence recovery after photobleaching (FRAP) and single-particle tracking (SPT) have to be applied to explore the dynamics of membrane components. The recently introduced raster image correlation spectroscopy (RICS) offers a much wider dynamic range than each of these methods separately and allows for spatial mapping of the dynamic properties. RICS is implemented on a confocal laser-scanning microscope (CLSM), and the wide dynamic range is achieved by exploiting the inherent time information carried by the scanning laser beam in the generation of the confocal images. The original introduction of RICS used two-photon excitation and photon counting detection. However, most CLSM systems are based on one-photon excitation with analog detection. Here we report on the performance of such a commercial CLSM (Zeiss LSM 510 META) in the study of the diffusion of the fluorescent lipid analog 1,1'-dioctadecyl-3,3,3',3'-tetramethyl-indodicarbocyanine perchlorate (DiI-C(18)(5)) both in giant unilamellar vesicles and in the plasma membrane of living oligodendrocytes, i.e., the myelin-producing cells of the central nervous system. It is shown that RICS on a commercial CLSM with analog detection allows for reliable results in the study of membrane diffusion by removal of unwanted correlations introduced by the analog detection system. The results obtained compare well with those collected by FRAP and FCS.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.