ArticleTAG. Theoretical and applied genetics. Theoretische und angewandte Genetik2009
Over-expression of aspartate aminotransferase genes in rice resulted in altered nitrogen metabolism and increased amino acid content in seeds.
Article in TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 52 papers.
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52 citing papers in PubMed, 140 citations in OpenAlex.
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- Divergence in the tomato rhizosphere microbial community structure driven by three soil types.Microbiology spectrum · 2026Article
- Optimization of carbon and nitrogen utilization for enhanced photosynthesis, biomass and yield by coexpression of phosphoenolpyruvate carboxylase, aspartate aminotransferase and glutamine synthetase in Brassica juncea.Plant biotechnology journal · 2026Article
- Exploring the Effects ofPlants (Basel, Switzerland) · 2025Article
- Identification and utilization of a novel large-effect QTL and linked markers for enhancing protein concentration in peanut.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2025Article
- Unlocking genetic hotspots: GWAS reveals key nitrogen responsive genomic regions and key genes for root and yield traits in indica rice.Molecular genetics and genomics : MGG · 2025Article
- From Gene to Plate: Molecular Insights into and Health Implications of Rice (International journal of molecular sciences · 2025Review
- Genome-Wide Analysis of the Aspartate Aminotransferase Family inInternational journal of molecular sciences · 2025Article
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- Genome-Wide Association and RNA-Seq Analyses Reveal a Potential Candidate Gene Related to Oil Content in Soybean Seeds.International journal of molecular sciences · 2024Article
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- Immune and physiological responses inFrontiers in immunology · 2024Article
- Genetically Modified Rice Is Associated with Hunger, Health, and Climate Resilience.Foods (Basel, Switzerland) · 2023Review
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- Effects of glutamate oxaloacetate transaminase on reactive oxygen species in Ganoderma lucidum.Applied microbiology and biotechnology · 2023Article
- Unlocking the potentials of nitrate transporters at improving plant nitrogen use efficiency.Frontiers in plant science · 2023Review
- Comparative Transcriptome and Co-Expression Network Analyses Reveal the Molecular Mechanism of Calcium-Deficiency-Triggered Tipburn in Chinese Cabbage (Plants (Basel, Switzerland) · 2022Article
- Transcriptome Analysis Reveals an Essential Role of Exogenous Brassinolide on the Alkaloid Biosynthesis Pathway in Pinellia Ternata.International journal of molecular sciences · 2022Article
- Deterioration in the Quality of RecalcitrantMetabolites · 2022Article
- New Insight into Aspartate Metabolic Pathways inInternational journal of molecular sciences · 2022Article
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6 authors at 1 institution in 1 country.
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No grant is acknowledged in the PubMed record.
Abstract
Aspartate aminotransferase (AAT) is a key enzyme in the synthesis of amino acids. It plays an important role in regulating carbon and nitrogen metabolism in almost all organisms. In this study, we over-expressed in rice separately all three AAT genes from rice (OsAAT1~3) and one AAT gene from Escherichia coli (EcAAT). Over-expression was driven by the CaMV 35S promoter and constructs were introduced into rice by Agrobacterium tumefaciens-mediated transformation. Compared with control plants, the transformants showed significantly increased leaf AAT activity and greater seed amino acid and protein contents. No other phenotypic changes were observed. The total leaf AAT activities in plants over-expressing OsAAT1, OsAAT2, and EcAAT were 26.6, 23.6, and 19.6 A min(-1) mg(-1) FW (A: units of activity, defined as increase of absorbency per min per mg; FW: fresh weight), which were significantly higher than that in the wild-type control (17.7 A min(-1) mg(-1) FW). The amino acid content in seeds of transgenic plants over-expressing OsAAT1, OsAAT2, and EcAAT was 119.36, 115.36, and 113.72 mg g(-1), respectively, which were 16.1, 12.0, and 5.4% higher, respectively, than that in the control plants. The transgenic plants over-expressing OsAAT1, OsAAT2, and EcAAT had significantly higher protein contents (increased 22.2, 21.1, and 11.1%, respectively) than wild-type plants. No significant changes were found in leaf AAT activity, seed amino acid content or protein content in OsAAT3 over-expressed plants. The expression patterns of the three OsAAT genes and their different functions are also discussed.
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