ArticleJournal of nematology2007
Quantitative Detection of Double-Stranded RNA-Mediated Gene Silencing of Parasitism Genes in Heterodera glycines.
Article in Journal of nematology, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
What it found
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Who cites it
15 citing papers in PubMed, 36 citations in OpenAlex.
- Nanoclay-based dsRNA delivery: a novel approach to control Globodera rostochiensis.Molecular biology reports · 2026Article
- The SUbventral-Gland Regulator (SUGR-1) of nematode virulence.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Comparative transcriptomics unravels new genes imparting scab resistance in apple (Malus x domestica Borkh.).Functional & integrative genomics · 2022Article
- A new fungus-mediated RNAi method established and used to study the fatty acid and retinol binding protein function of the plant-parasitic nematodeRNA biology · 2021Article
- Quantification of polyphenolic compounds and relative gene expression studies of phenylpropanoid pathway in apple (Saudi journal of biological sciences · 2020Article
- Inhibition of acetyl-CoA carboxylase by spirotetramat causes growth arrest and lipid depletion in nematodes.Scientific reports · 2020Article
- Liposome-based transfection enhances RNAi and CRISPR-mediated mutagenesis in non-model nematode systems.Scientific reports · 2019Article
- An Effector from the Cyst NematodeThe Plant cell · 2018Article
- Novel Pectate Lyase Genes of Heterodera glycines Play Key Roles in the Early Stage of Parasitism.PloS one · 2016Article
- A parasitic nematode releases cytokinin that controls cell division and orchestrates feeding site formation in host plants.Proceedings of the National Academy of Sciences of the United States of America · 2015Article
- Development of an in vitro model system for studying bacterially expressed dsRNA-mediated knockdown in Neoparamoeba genus.Marine biotechnology (New York, N.Y.) · 2014Article
- Several Grassland Soil Nematode Species Are Insensitive to RNA-Mediated Interference.Journal of nematology · 2012Article
- Expression profiling and cross-species RNA interference (RNAi) of desiccation-induced transcripts in the anhydrobiotic nematode Aphelenchus avenae.BMC molecular biology · 2010Article
- Characterization of a putative endoxylanase in the migratory plant-parasitic nematode Radopholus similis.Molecular plant pathology · 2009Article
- Article
Corrections and comments
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Authors and funding
8 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The introduction of a double-stranded RNA (dsRNA) into an organism to induce sequence-specific RNA interference (RNAi) of a target transcript has become a powerful technique to investigate gene function in nematodes and many organisms. Data provided here indicate that the inclusion of 1-2 mM spermidine and 50 mM octopamine and a 24 hr incubation period of nematodes in double-stranded RNA (dsRNA) soaking solutions resulted in a considerable increase in the percentage of nematodes that ingested dsRNA as compared to previous reports. This modified dsRNA soaking method was coupled with quantitative real-time RT-PCR (qRT-PCR) analyses to assess the potential silencing of the Heterodera glycines parasitism gene transcripts Hg-pel-1 and Hg-4E02 that are expressed within the esophageal gland cells of preparasitic H. glycines J2. The Hg-pel-1 transcript was most efficiently silenced with one dsRNA construct (ds267) at the highest dsRNA soaking concentration of 5.0 mg/ml, while the Hg-4E02 transcript was more efficiently silenced at the 2.5 mg/ml dsRNA concentration as compared to 5.0 mg/ml. A dsRNA construct (ds285) complementary to a different sequence within the Hg-pel-1 transcript than construct ds267 induced only minimal silencing of the Hg-pel-1 transcript at 2.5 mg/ml. The results suggest that both dsRNA concentration and sequence relative to the transcript targeted are critical for maximizing potential RNAi effects in parasitic nematodes.
Identifiers
19259483PMC2586495W2155433914What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.