ArticleCell research2009
Repression of PKR mediates palmitate-induced apoptosis in HepG2 cells through regulation of Bcl-2.
Article in Cell research, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 38 citations in OpenAlex.
- Eukaryotic translation initiation factor 2α kinase 2 in pancreatic cancer: An approach towards managing clinical prognosis and molecular immunological characterization.Oncology letters · 2023Article
- GRP78/BiP alleviates oxLDL-induced hepatotoxicity in familial hypercholesterolemia caused by missense variants of LDLR in a HepG2 cellular model.Lipids in health and disease · 2023Article
- Phosphorylation of BCL2 at the Ser70 site mediates RANKL-induced osteoclast precursor autophagy and osteoclastogenesis.Molecular medicine (Cambridge, Mass.) · 2022Article
- The importance and future of biochemical engineering.Biotechnology and bioengineering · 2020Article
- A secretome profile indicative of oleate-induced proliferation of HepG2 hepatocellular carcinoma cells.Experimental & molecular medicine · 2018Article
- Interferon-α inhibits cell migration and invasion and induces the expression of antiviral proteins in Huh-7 cells transfected with hepatitis B virus X gene-expressing lentivirus.Experimental and therapeutic medicine · 2017Article
- Contribution of C1485T mutation in the HBx gene to human and murine hepatocarcinogenesis.Scientific reports · 2017Article
- Sustained Action of Ceramide on the Insulin Signaling Pathway in Muscle Cells: IMPLICATION OF THE DOUBLE-STRANDED RNA-ACTIVATED PROTEIN KINASE.The Journal of biological chemistry · 2016Article
- Double stranded RNA-dependent protein kinase promotes the tumorigenic phenotype in HepG2 hepatocellular carcinoma cells by activating STAT3.Oncology letters · 2014Article
- Improved asymmetry prediction for short interfering RNAs.The FEBS journal · 2014Article
- DNA methyltransferase inhibitor zebularine inhibits human hepatic carcinoma cells proliferation and induces apoptosis.PloS one · 2013Article
- Endoplasmic reticulum stress and the unfolded protein response in nonalcoholic fatty liver disease.Antioxidants & redox signaling · 2011Review
- Molecular mechanism by which palmitate inhibits PKR autophosphorylation.Biochemistry · 2011Article
- The double-stranded RNA-dependent protein kinase differentially regulates insulin receptor substrates 1 and 2 in HepG2 cells.Molecular biology of the cell · 2010Article
- DNA damage and DNA damage responses in THP-1 monocytes after exposure to spores of either Stachybotrys chartarum or Aspergillus versicolor or to T-2 toxin.Toxicological sciences : an official journal of the Society of Toxicology · 2010Article
- A dynamic analysis of IRS-PKR signaling in liver cells: a discrete modeling approach.PloS one · 2009Article
- Reconstruct modular phenotype-specific gene networks by knowledge-driven matrix factorization.Bioinformatics (Oxford, England) · 2009Article
- The role of fatty acid unsaturation in minimizing biophysical changes on the structure and local effects of bilayer membranes.Biochimica et biophysica acta · 2009Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
The present study shows that double-stranded RNA-dependent protein kinase (PKR) regulates the protein expression level and phosphorylation of Bcl-2 and plays an anti-apoptotic role in human hepatocellular carcinoma cells (HepG2). In various types of cells, saturated free fatty acids (FFAs), such as palmitate, have been shown to induce cellular apoptosis by several mechanisms. Palmitate down-regulates the activity of PKR and thereby decreases the level of Bcl-2 protein, mediated in part by reduced activation of the NF-kappaB transcription factor. In addition to the level of Bcl-2 protein, the phosphorylation of Bcl-2 at different amino acid residues, such as Ser70 and Ser87, is also important in regulating cellular apoptosis. The decrease in the phosphorylation of Bcl-2 at Ser70 upon exposure to palmitate is mediated by inhibition of PKR and possibly by c-Jun N-terminal kinase (JNK), whereas the phosphorylation of Bcl-2 at Ser87 is unaffected by palmitate or PKR. In summary, PKR mediates the regulation of the protein level and the phosphorylation status of Bcl-2, providing a novel mechanism of palmitate-induced apoptosis in HepG2 cells.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.