ArticleAnalytical biochemistry2009
Chemically modified primers for improved multiplex polymerase chain reaction.
Article in Analytical biochemistry, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
6 citing papers in PubMed.
- Performance evaluation and application of a multiplex PCR capillary electrophoresis method for detecting nucleic acids of seven sexually transmitted pathogens.Frontiers in cellular and infection microbiology · 2026Article
- Multicenter clinical performance and comparative evaluation of the LIAISON PLEX RespiratoryJournal of clinical microbiology · 2025Article
- Nanoparticle-assisted PCR: fundamentals, mechanisms, and forensic implications.International journal of legal medicine · 2025Review
- Malaria diagnostic update: From conventional to advanced method.Journal of clinical laboratory analysis · 2022Review
- DNA Microsystems for Biodiagnosis.Micromachines · 2020Review
- PCR Strategies for Complete Allele Calling in Multigene Families Using High-Throughput Sequencing Approaches.PloS one · 2016Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors.
Funding
Abstract
Multiplex polymerase chain reaction (PCR), the amplification of multiple targets in a single reaction, presents a new set of challenges that further complicate more traditional PCR setups. These complications include a greater probability for nonspecific amplicon formation and for imbalanced amplification of different targets, each of which can compromise quantification and detection of multiple targets. Despite these difficulties, multiplex PCR is frequently used in applications such as pathogen detection, RNA quantification, mutation analysis, and (recently) next generation DNA sequencing. Here we investigated the utility of primers with one or two thermolabile 4-oxo-1-pentyl phosphotriester modifications in improving multiplex PCR performance. Initial endpoint and real-time analyses revealed a decrease in off-target amplification and a subsequent increase in amplicon yield. Furthermore, the use of modified primers in multiplex setups revealed a greater limit of detection and more uniform amplification of each target as compared with unmodified primers. Overall, the thermolabile modified primers present a novel and exciting avenue for improving multiplex PCR performance.
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Registered trials
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