ArticleBMC plant biology2009
Characterization of PR-10 genes from eight Betula species and detection of Bet v 1 isoforms in birch pollen.
Article in BMC plant biology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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19 citing papers in PubMed, 60 citations in OpenAlex.
- Natural killer T cells in allergic asthma: implications for the development of novel immunotherapeutical strategies.Frontiers in immunology · 2024Review
- Identification and analysis of major latex protein (Frontiers in plant science · 2024Article
- Revealing the contribution of GbPR10.5D1 to resistance against Verticillium dahliae and its regulation for structural defense and immune signaling.The plant genome · 2022Article
- Identification of allergenomic signatures in allergic and well-tolerated apple genotypes using LC-MS/MS.Food chemistry. Molecular sciences · 2022Article
- Microscale Thermophoresis Reveals Oxidized Glutathione as High-Affinity Ligand of Mal d 1.Foods (Basel, Switzerland) · 2021Article
- Tiered approach for the identification of Mal d 1 reduced, well tolerated apple genotypes.Scientific reports · 2020Article
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- Six years of INSTAND e. V. sIgE proficiency testing: An evaluation of in vitro allergy diagnostics.Allergo journal international · 2017Article
- Moss Pathogenesis-Related-10 Protein Enhances Resistance to Pythium irregulare in Physcomitrella patens and Arabidopsis thaliana.Frontiers in plant science · 2016Article
- Molecular Cloning and Expression Analysis of hyp-1 Type PR-10 Family Genes in Hypericum perforatum.Frontiers in plant science · 2016Article
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- Quantitative DNA Analyses for Airborne Birch Pollen.PloS one · 2015Article
- Article
- Pathogenesis related-10 proteins are small, structurally similar but with diverse role in stress signaling.Molecular biology reports · 2014Review
- Current overview of allergens of plant pathogenesis related protein families.TheScientificWorldJournal · 2014Review
- A qRT-PCR assay for the expression of all Mal d 1 isoallergen genes.BMC plant biology · 2013Article
- Characterisation of the Vitis vinifera PR10 multigene family.BMC plant biology · 2010Article
- Scientific Opinion on the assessment of allergenicity of GM plants and microorganisms and derived food and feed.EFSA journal. European Food Safety Authority · 2010Article
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Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundBet v 1 is an important cause of hay fever in northern Europe. Bet v 1 isoforms from the European white birch (Betula pendula) have been investigated extensively, but the allergenic potency of other birch species is unknown. The presence of Bet v 1 and closely related PR-10 genes in the genome was established by amplification and sequencing of alleles from eight birch species that represent the four subgenera within the genus Betula. Q-TOF LC-MSE was applied to identify which PR-10/Bet v 1 genes are actually expressed in pollen and to determine the relative abundances of individual isoforms in the pollen proteome.
resultsAll examined birch species contained several PR-10 genes. In total, 134 unique sequences were recovered. Sequences were attributed to different genes or pseudogenes that were, in turn, ordered into seven subfamilies. Five subfamilies were common to all birch species. Genes of two subfamilies were expressed in pollen, while each birch species expressed a mixture of isoforms with at least four different isoforms. Isoforms that were similar to isoforms with a high IgE-reactivity (Bet v 1a = PR-10.01A01) were abundant in all species except B. lenta, while the hypoallergenic isoform Bet v 1d (= PR-10.01B01) was only found in B. pendula and its closest relatives.
conclusionQ-TOF LC-MSE allows efficient screening of Bet v 1 isoforms by determining the presence and relative abundance of these isoforms in pollen. B. pendula contains a Bet v 1-mixture in which isoforms with a high and low IgE-reactivity are both abundant. With the possible exception of B. lenta, isoforms identical or very similar to those with a high IgE-reactivity were found in the pollen proteome of all examined birch species. Consequently, these species are also predicted to be allergenic with regard to Bet v 1 related allergies.
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