ArticleLab on a chip2009
Rapid generation of spatially and temporally controllable long-range concentration gradients in a microfluidic device.
Article in Lab on a chip, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
26 citing papers in PubMed.
- Spatial regulation of hydrogel polymerization reaction using ultrasound-driven streaming vortex.Ultrasonics sonochemistry · 2024Article
- Advances in passively driven microfluidics and lab-on-chip devices: a comprehensive literature review and patent analysis.RSC advances · 2020Review
- In vitro metabolic zonation through oxygen gradient on a chip.Scientific reports · 2019Article
- A simple layer-stacking technique to generate biomolecular and mechanical gradients in photocrosslinkable hydrogels.Biofabrication · 2019Article
- Flow characterization and patch clamp dose responses using jet microfluidics in a tubeless microfluidic device.Journal of neuroscience methods · 2017Article
- 3D Microtissues for Injectable Regenerative Therapy and High-throughput Drug Screening.Journal of visualized experiments : JoVE · 2017Article
- Generating 2-dimensional concentration gradients of biomolecules using a simple microfluidic design.Biomicrofluidics · 2017Article
- Humidity assay for studying plant-pathogen interactions in miniature controlled discrete humidity environments with good throughput.Biomicrofluidics · 2016Article
- The effect of delivering the chemokine SDF-1α in a matrix-bound manner on myogenesis.Biomaterials · 2014Article
- Spatial patterning of BMP-2 and BMP-7 on biopolymeric films and the guidance of muscle cell fate.Biomaterials · 2014Article
- Gradients of physical and biochemical cues on polyelectrolyte multilayer films generated via microfluidics.Lab on a chip · 2013Article
- An integrated microfluidic device for two-dimensional combinatorial dilution.Lab on a chip · 2011Article
- Anisotropic material synthesis by capillary flow in a fluid stripe.Biomaterials · 2011Article
- Integrated microfluidic array plate (iMAP) for cellular and molecular analysis.Lab on a chip · 2011Article
- Drug-eluting microarrays for cell-based screening of chemical-induced apoptosis.Analytical chemistry · 2011Article
- A microfluidic-based neurotoxin concentration gradient for the generation of an in vitro model of Parkinson's disease.Biomicrofluidics · 2011Article
- Surface-tension-driven gradient generation in a fluid stripe for bench-top and microwell applications.Small (Weinheim an der Bergstrasse, Germany) · 2011Article
- Generating nonlinear concentration gradients in microfluidic devices for cell studies.Analytical chemistry · 2011Article
- Microfluidic synthesis of composite cross-gradient materials for investigating cell-biomaterial interactions.Biotechnology and bioengineering · 2011Article
- A low resistance microfluidic system for the creation of stable concentration gradients in a defined 3D microenvironment.Biomedical microdevices · 2010Article
Corrections and comments
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Authors and funding
10 authors.
Funding
Abstract
The ability to rapidly generate concentration gradients of diffusible molecules has important applications in many chemical and biological studies. Here we established spatially and temporally controllable concentration gradients of molecules (i.e. proteins or toxins) in a portable microfluidic device in an easy and rapid manner. The formation of the concentration gradients was initiated by a passive-pump-induced forward flow and further optimized during an evaporation-induced backward flow. The centimeter-long gradients along the microfluidic channel were shown to be spatially and temporally controlled by the backward flow. The gradient profile was stabilized by stopping the flow. Computational simulations of this dynamic process illustrated the combined effects of convection and diffusion on the gradient generation, and fit well with the experimental data. To demonstrate the applications of this methodology, a stabilized concentration gradient of a cardiac toxin, alpha-cypermethrin, along the microchannel was used to test the response of HL-1 cardiac cells in the micro-device, which correlated with toxicity data obtained from multi-well plates. The approach presented here may be useful for many biological and chemical processes that require rapid generation of long-range gradients in a portable microfluidic device.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.