ArticleJournal of veterinary science2009
A multiplex real-time PCR for differential detection and quantification of Salmonella spp., Salmonella enterica serovar Typhimurium and Enteritidis in meats.
Article in Journal of veterinary science, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed.
- Meat-Borne Bacterial Pathogen Detection: Conventional, Molecular and Emerging AI-Based Strategies.Diagnostics (Basel, Switzerland) · 2026Review
- Evaluation of mdh, dld, tcfA, and folE gene markers for detection of enteric fever using real-time PCR.Scientific reports · 2026Article
- Polymerase Chain Reaction: A Toolbox for Molecular Discovery.Molecular biotechnology · 2026Review
- Electrospun Nanofiber Platforms for Advanced Sensors in Livestock-Derived Food Quality and Safety Monitoring: A Review.Sensors (Basel, Switzerland) · 2025Review
- Nanofiber-Enabled Rapid and Non-Destructive Sensors for Meat Quality and Shelf-Life Monitoring: A Review.Foods (Basel, Switzerland) · 2025Review
- Development and Evaluation of a Next-Generation Sequencing Panel for the Multiple Detection and Identification of Pathogens in Fermented Foods.Journal of microbiology and biotechnology · 2023Article
- Biofilm formation of Salmonella enterica serovar Enteritidis cocultured with Acanthamoeba castellanii responds to nutrient availability.International microbiology : the official journal of the Spanish Society for Microbiology · 2022Article
- Molecular Typing, Antibiotic Resistance and Enterotoxin Gene Profiles ofMicroorganisms · 2022Article
- The protective effect of dietary supplementation ofJournal of advanced veterinary and animal research · 2021Article
- Cross-kingdom inhibition of bacterial virulence and communication by probiotic yeast metabolites.Microbiome · 2021Article
- Rapid Detection and Differentiating of the PredominantFrontiers in cellular and infection microbiology · 2021Article
- Modulations of growth performance, gut microbiota, and inflammatory cytokines by trehalose on Salmonella Typhimurium-challenged broilers.Poultry science · 2020Article
- Combined effects of3 Biotech · 2020Article
- Resistance Profiles ofFrontiers in microbiology · 2020Article
- Short Chain Fatty Acids Commonly Produced by Gut Microbiota InfluenceAntibiotics (Basel, Switzerland) · 2019Article
- A rapid colorimetric immunoassay for the detection of pathogenic bacteria on poultry processing plants using cotton swabs and nanobeads.Mikrochimica acta · 2018Article
- Article
- Prevalence ofVeterinary world · 2017Article
- Water Access, Sanitation, and Hygiene Conditions and Health Outcomes among Two Settlement Types in Rural Far North Cameroon.International journal of environmental research and public health · 2017Article
- Development of a novel detection system for microbes from bovine diarrhea by real-time PCR.The Journal of veterinary medical science · 2016Article
Corrections and comments
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Authors and funding
8 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Salmonella (S.) Typhimurium and S. Enteritidis are the major causative agents of food-borne illnesses worldwide. Currently, a rapid detection system using multiplex real-time polymerase chain reaction (PCR) has been applied for other food-borne pathogens such as Escherichia coli, Staphylococcus aureus and Streptococcus spp. A multiplex real-time PCR was developed for the simultaneous detection of Salmonella spp., especially S. Typhimurium and S. Enteritidis, in beef and pork. For the specific and sensitive multiplex real-time PCR, three representative primers and probes were designed based on sequence data from Genbank. Among the three DNA extraction methods (boiling, alkaline lysis, and QIAamp DNA Mini Kit), the QIAamp DNA Mini Kit was the most sensitive in this study. The optimized multiplex real-time PCR was applied to artificially inoculated beef or pork. The detection sensitivity of the multiplex real-time PCR was increased. The specificity of the multiplex real-time PCR assay, using 128 pure-cultured bacteria including 110 Salmonella isolates and 18 non-Salmonella isolates, was 100%, 100% and 99.1% for Salmonella spp., S. Typhimurium and S. Enteritidis, respectively. The sensitivity was 100%, 100% and 91.7% for Salmonella spp., S. Typhimurium and S. Enteritidis, respectively. The multiplex real-time PCR assay developed in this study could detect up to 0.54 +/- 0.09 and 0.65 +/- 0.07 log(10) CFU/ml for S. Typhimurium and S. Enteritidis for beef, 1.45 +/- 0.21 and 1.65 +/- 0.07 log(10) CFU/ml for S. Typhimurium and S. Enteritidis for pork, respectively, with all conditions optimized. Our results indicated that the multiplex real-time PCR assay developed in this study could sensitively detect Salmonella spp. and specifically differentiate S. Typhimurium from S. Enteritidis in meats.
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