ArticleJournal of veterinary science2009
A biosensor assay for the detection of Mycobacterium avium subsp. paratuberculosis in fecal samples.
Article in Journal of veterinary science, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.
What it found
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The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
6 citing papers in PubMed, 43 citations in OpenAlex.
- Detection of Mycobacterium avium subsp. paratuberculosis in Bovine Faeces Using a Rhodamine B Hydrazone Immunosensor on Antibody-Coated Nitrocellulose Membranes.Veterinary medicine and science · 2026Article
- Recent Advances in Biosensor Technologies for Meat Production Chain.Foods (Basel, Switzerland) · 2025Review
- Membrane adhesion and the formation of heterogeneities: biology, biophysics, and biotechnology.Physical chemistry chemical physics : PCCP · 2015Review
- A Review of Membrane-Based Biosensors for Pathogen Detection.Sensors (Basel, Switzerland) · 2015Review
- Comparison between a conductometric biosensor and ELISA in the evaluation of Johne's disease.Sensors (Basel, Switzerland) · 2014Article
- Development of chemiluminescent lateral flow assay for the detection of nucleic acids.Biosensors · 2012Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A simple, membrane-strip-based lateral-flow (LF) biosensor assay and a high-throughput microtiter plate assay have been combined with a reverse transcriptase polymerase chain reaction (RT-PCR) for the detection of a small number (ten) of viable Mycobacterium (M.) avium subsp. paratuberculosis (MAP) cells in fecal samples. The assays are based on the identification of the RNA of the IS900 element of MAP. For the assay, RNA was extracted from fecal samples spiked with a known quantity of (10(1) to 10(6)) MAP cells and amplified using RT-PCR and identified by the LF biosensor and the microtiter plate assay. While the LF biosensor assay requires only 30 min of assay time, the overall process took 10 h for the detection of 10 viable cells. The assays are based on an oligonucleotide sandwich hybridization assay format and use either a membrane flow through system with an immobilized DNA probe that hybridizes with the target sequence or a microtiter plate well. Signal amplification is provided when the target sequence hybridizes to a second DNA probe that has been coupled to liposomes encapsulating the dye, sulforhodamine B. The dye in the liposomes provides a signal that can be read visually, quantified with a hand-held reflectometer, or with a fluorescence reader. Specificity analysis of the assays revealed no cross reactivity with other mycobacteria, such as M. avium complex, M. ulcerans, M. marium, M. kansasii, M. abscessus, M. asiaticum, M. phlei, M. fortutitum, M. scrofalaceum, M. intracellular, M. smegmatis, and M. bovis. The overall assay for the detection of live MAP organisms is comparatively less expensive and quick, especially in comparison to standard MAP detection using a culture method requiring 6-8 weeks of incubation time, and is significantly less expensive than real-time PCR.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.