ArticleActa crystallographica. Section F, Structural biology and crystallization communications2009
Expression, purification and crystallization of class C acid phosphatases from Francisella tularensis and Pasteurella multocida.
Article in Acta crystallographica. Section F, Structural biology and crystallization communications, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.
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3 citing papers in PubMed, 3 citations in OpenAlex.
- Structural basis of the inhibition of class C acid phosphatases by adenosine 5'-phosphorothioate.The FEBS journal · 2011Article
- Crystal structure and immunogenicity of the class C acid phosphatase from Pasteurella multocida.Archives of biochemistry and biophysics · 2011Article
- Crystal Structures of the histidine acid phosphatase from Francisella tularensis provide insight into substrate recognition.Journal of molecular biology · 2009Article
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Authors and funding
7 authors at 1 institution in 1 country.
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Abstract
Class C nonspecific acid phosphatases are bacterial enzymes that are secreted across the cytoplasmic membrane and hydrolyze a variety of phosphomonoesters at acidic pH. These enzymes are of interest for the development of improved vaccines and clinical diagnostic methods. In one case, the category A pathogen Francisella tularensis, the class C phosphatase plays a role in bacterial fitness. Here, the cloning, expression, purification and crystallization methods for the class C acid phosphatases from F. tularensis and Pasteurella multocida are reported. Crystals of the F. tularensis enzyme diffracted to 2.0 A resolution and belonged to space group C222(1), with one enzyme molecule in the asymmetric unit. Crystals of the P. multocida enzyme diffracted to 1.85 A resolution and belonged to space group C2, with three molecules in the asymmetric unit. Diffraction patterns from crystals of the P. multocida enzyme exhibited multiple interpenetrating reciprocal-space lattices, indicating epitaxial twinning. Despite this aberrance, autoindexing was robust and the data could be satisfactorily processed to 1.85 A resolution using MOSFLM and SCALA.
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