ArticleActa crystallographica. Section F, Structural biology and crystallization communications2009
Crystallization and preliminary X-ray diffraction analysis of the lectin from Canavalia boliviana Piper seeds.
Article in Acta crystallographica. Section F, Structural biology and crystallization communications, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
3 citing papers in PubMed.
- Structural studies of an anti-inflammatory lectin from Canavalia boliviana seeds in complex with dimannosides.PloS one · 2014Article
- Effect of lectins from Diocleinae subtribe against oral Streptococci.Molecules (Basel, Switzerland) · 2011Article
- Antinociceptive activity and toxicology of the lectin from Canavalia boliviana seeds in mice.Naunyn-Schmiedeberg's archives of pharmacology · 2009Article
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Authors and funding
11 authors.
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No grant is acknowledged in the PubMed record.
Abstract
Plant lectins are the most studied group of carbohydrate-binding proteins. Despite the high similarity between the members of the Diocleinae subtribe (Leguminosae) group, they present differing biological activities. Canavalia boliviana lectin (Cbol) was purified using a Sephadex G-50 column and crystallized in the presence of X-Man by hanging-drop vapour diffusion at 293 K. After optimization, crystals suitable for diffraction were obtained under the condition 0.1 M HEPES pH 7.5 and 3.0 M sodium formate. The crystal belonged to the monoclinic space group C2, with unit-cell parameters a = 126.70, b = 66.64, c = 64.99 A, alpha = 90.0, beta = 120.8, gamma = 90.0 degrees . Assuming the presence of a dimer in the asymmetric unit, the solvent content was estimated to be about 46%. A complete data set was collected at 1.5 A resolution.
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