ArticleJournal of magnetic resonance (San Diego, Calif. : 1997)2009
Determining the helical tilt of membrane peptides using electron paramagnetic resonance spectroscopy.
Article in Journal of magnetic resonance (San Diego, Calif. : 1997), 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
What it found
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
10 citing papers in PubMed.
- Studies of transmembrane peptides by pulse dipolar spectroscopy with semi-rigid TOPP spin labels.European biophysics journal : EBJ · 2021Review
- Probing topology and dynamics of the second transmembrane domain (M2δ) of the acetyl choline receptor using magnetically aligned lipid bilayers (bicelles) and EPR spectroscopy.Chemistry and physics of lipids · 2017Article
- Probing the Secondary Structure of Membrane Peptides Using (2)H-Labeled d(10)-Leucine via Site-Directed Spin-Labeling and Electron Spin Echo Envelope Modulation Spectroscopy.The journal of physical chemistry. B · 2016Article
- Peptide-Membrane Interactions by Spin-Labeling EPR.Methods in enzymology · 2015Article
- Solid-state NMR (31)P paramagnetic relaxation enhancement membrane protein immersion depth measurements.The journal of physical chemistry. B · 2014Article
- When detergent meets bilayer: birth and coming of age of lipid bicelles.Progress in nuclear magnetic resonance spectroscopy · 2013Review
- The magic of bicelles lights up membrane protein structure.Chemical reviews · 2012Review
- Determining the orientation and localization of membrane-bound peptides.Current protein & peptide science · 2012Review
- The spin label amino acid TOAC and its uses in studies of peptides: chemical, physicochemical, spectroscopic, and conformational aspects.Biophysical reviews · 2012Article
- Solid-state NMR paramagnetic relaxation enhancement immersion depth studies in phospholipid bilayers.Journal of magnetic resonance (San Diego, Calif. : 1997) · 2010Article
Corrections and comments
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Authors and funding
5 authors.
Funding
Abstract
Theoretical calculations of hyperfine splitting values derived from the EPR spectra of TOAC spin-labeled rigid aligned alpha-helical membrane peptides reveal a unique periodic variation. In the absence of helical motion, a plot of the corresponding hyperfine splitting values as a function of residue number results in a sinusoidal curve that depends on the helical tilt angle that the peptide makes with respect to the magnetic field. Motion about the long helical axis reduces the amplitude of the curve and averages out the corresponding hyperfine splitting values. The corresponding spectra can be used to determine the director axis tilt angle from the TOAC spin label, which can be used to calculate the helical tilt angle due to the rigidity of the TOAC spin label. Additionally, this paper describes a method to experimentally determine this helical tilt angle from the hyperfine splitting values of three consecutive residues.
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Registered trials
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