ArticleDevelopmental biology2009
The bHLH transcription factor Hand2 is essential for the maintenance of noradrenergic properties in differentiated sympathetic neurons.
Article in Developmental biology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers.
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Who cites it
35 citing papers in PubMed, 65 citations in OpenAlex.
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- A knock-in allele of Hand2 expressing Dre recombinase.Genesis (New York, N.Y. : 2000) · 2024Article
- Maintenance of neuronal fate and transcriptional identity.Biology open · 2023Review
- Somatic Cell Reprogramming for Nervous System Diseases: Techniques, Mechanisms, Potential Applications, and Challenges.Brain sciences · 2023Review
- Linking human sympathoadrenal development and neuroblastoma.Nature genetics · 2021Article
- The emerging role of the sympathetic nervous system in skeletal muscle motor innervation and sarcopenia.Ageing research reviews · 2021Review
- The diversity of neuronal phenotypes in rodent and human autonomic ganglia.Cell and tissue research · 2020Review
- Transcription Factors Phox2a/2b Upregulate Expression of Noradrenergic and Dopaminergic Phenotypes in Aged Rat Brains.Neurotoxicity research · 2020Article
- Striking parallels between carotid body glomus cell and adrenal chromaffin cell development.Developmental biology · 2018Article
- Sympathetic tales: subdivisons of the autonomic nervous system and the impact of developmental studies.Neural development · 2018Review
- Significant transcriptional changes in 15q duplication but not Angelman syndrome deletion stem cell-derived neurons.Molecular autism · 2018Article
- Part and Parcel of the Cardiac Autonomic Nerve System: Unravelling Its Cellular Building Blocks during Development.Journal of cardiovascular development and disease · 2016Review
- Islet 1 specifies the identity of hypothalamic melanocortin neurons and is critical for normal food intake and adiposity in adulthood.Proceedings of the National Academy of Sciences of the United States of America · 2015Article
- Satb2-independent acquisition of the cholinergic sudomotor phenotype in rodents.Cellular and molecular neurobiology · 2015Article
- The bHLH Transcription Factor Hand Regulates the Expression of Genes Critical to Heart and Muscle Function in Drosophila melanogaster.PloS one · 2015Article
- Maintenance of postmitotic neuronal cell identity.Nature neuroscience · 2014Review
- Loss of Hand2 in a population of Periostin lineage cells results in pronounced bradycardia and neonatal death.Developmental biology · 2014Article
- The LIM-Homeodomain transcription factor Islet-1 is required for the development of sympathetic neurons and adrenal chromaffin cells.Developmental biology · 2013Article
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Authors and funding
8 authors at 4 institutions in 3 countries.
Funding
Abstract
The basic helix-loop-helix transcription factor Hand2 is essential for the proliferation and noradrenergic differentiation of sympathetic neuron precursors during development. Here we address the function of Hand2 in postmitotic, differentiated sympathetic neurons. Knockdown of endogenous Hand2 in cultured E12 chick sympathetic neurons by siRNA results in a significant (about 60%) decrease in the expression of the noradrenergic marker genes dopamine-beta-hydroxylase (DBH) and tyrosine hydroxylase (TH). In contrast, expression of the pan-neuronal genes TuJ1, HuC and SCG10 was not affected. To analyze the in vivo role of Hand2 in differentiated sympathetic neurons we used mice harboring a conditional Hand2-null allele and excised the gene by expression of Cre recombinase under control of the DBH promotor. Mouse embryos homozygous for Hand2 gene deletion showed decreased sympathetic neuron number and TH expression was strongly reduced in the residual neuron population. The in vitro Hand2 knockdown also enhances the CNTF-induced expression of the cholinergic marker genes vesicular acetylcholine transporter (VAChT) and choline acetyltransferase (ChAT). Taken together, these findings demonstrate that the Hand2 transcription factor plays a key role in maintaining noradrenergic properties in differentiated neurons.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.