ArticleBiophysical journal2009
Prestress and adhesion site dynamics control cell sensitivity to extracellular stiffness.
Article in Biophysical journal, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 42 citations in OpenAlex.
- Photonic-crystal hydraulic manometry quantifies epithelial basal compression for rapid, label-free functional screening.Science advances · 2026Article
- A comprehensive study of AFM stiffness measurements on inclined surfaces: theoretical, numerical, and experimental evaluation using a Hertz approach.Scientific reports · 2024Article
- Nanoscale mechanical properties of chitosan hydrogels as revealed by AFM.Progress in biomaterials · 2020Article
- Molecular regulation of TLR signaling in health and disease: mechano-regulation of macrophages and TLR signaling.Innate immunity · 2020Review
- Toll-like receptor signaling in macrophages is regulated by extracellular substrate stiffness and Rho-associated coiled-coil kinase (ROCK1/2).International immunology · 2018Article
- Keloid progression: a stiffness gap hypothesis.International wound journal · 2017Article
- Cellular level robotic surgery: Nanodissection of intermediate filaments in live keratinocytes.Nanomedicine : nanotechnology, biology, and medicine · 2015Article
- Influence of airway wall compliance on epithelial cell injury and adhesion during interfacial flows.Journal of applied physiology (Bethesda, Md. : 1985) · 2014Article
- Integrin-matrix clusters form podosome-like adhesions in the absence of traction forces.Cell reports · 2013Article
- Modulation of Nuclear Shape by Substrate Rigidity.Cellular and molecular bioengineering · 2013Article
- Geometry sensing by dendritic cells dictates spatial organization and PGE(2)-induced dissolution of podosomes.Cellular and molecular life sciences : CMLS · 2012Article
- The effects of substrate stiffness on the in vitro activation of macrophages and in vivo host response to poly(ethylene glycol)-based hydrogels.Journal of biomedical materials research. Part A · 2012Article
- Article
- Polymer microlenses for quantifying cell sheet mechanics.Soft matter · 2010Article
- Actin machinery and mechanosensitivity in invadopodia, podosomes and focal adhesions.Journal of cell science · 2009Review
Corrections and comments
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Authors and funding
9 authors at 5 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
This study aims at improving the understanding of mechanisms responsible for cell sensitivity to extracellular environment. We explain how substrate mechanical properties can modulate the force regulation of cell sensitive elements primarily adhesion sites. We present a theoretical and experimental comparison between two radically different approaches of the force regulation of adhesion sites that depends on their either stationary or dynamic behavior. The most classical stationary model fails to predict cell sensitivity to substrate stiffness whereas the dynamic model predicts extracellular stiffness dependence. This is due to a time dependent reaction force in response to actomyosin traction force exerted on cell sensitive elements. We purposely used two cellular models, i.e., alveolar epithelial cells and alveolar macrophages exhibiting respectively stationary and dynamic adhesion sites, and compared their sensitivity to theoretical predictions. Mechanical and structural results show that alveolar epithelial cells exhibit significant prestress supported by evident stress fibers and lacks sensitivity to substrate stiffness. On the other hand, alveolar macrophages exhibit low prestress and exhibit sensitivity to substrate stiffness. Altogether, theory and experiments consistently show that adhesion site dynamics and cytoskeleton prestress control cell sensitivity to extracellular environment with an optimal sensitivity expected in the intermediate range.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.