ArticleBiophysical journal2009
A nebulin ruler does not dictate thin filament lengths.
Article in Biophysical journal, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 60 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
60 citing papers in PubMed, 86 citations in OpenAlex.
- Independent pathways control muscle tissue size and sarcomere remodeling.Developmental biology · 2022Article
- The Mechanisms of Thin Filament Assembly and Length Regulation in Muscles.International journal of molecular sciences · 2022Review
- Article
- Diversification of the calponin family proteins by gene amplification and repeat expansion of calponin-like motifs.Cytoskeleton (Hoboken, N.J.) · 2021Article
- Nebulin and Lmod2 are critical for specifying thin-filament length in skeletal muscle.Science advances · 2020Article
- Nebulin: big protein with big responsibilities.Journal of muscle research and cell motility · 2020Review
- Assembly and Maintenance of Sarcomere Thin Filaments and Associated Diseases.International journal of molecular sciences · 2020Review
- The complexity of titin splicing pattern in human adult skeletal muscles.Skeletal muscle · 2018Article
- Overview of the Muscle Cytoskeleton.Comprehensive Physiology · 2017Review
- Tropomodulins and Leiomodins: Actin Pointed End Caps and Nucleators in Muscles.Biophysical journal · 2017Review
- Software-based measurement of thin filament lengths: an open-source GUI for Distributed Deconvolution analysis of fluorescence images.Journal of microscopy · 2017Article
- Aberrant developmental titin splicing and dysregulated sarcomere length in Thymosin β4 knockout mice.Journal of molecular and cellular cardiology · 2017Article
- Thin filament length in the cardiac sarcomere varies with sarcomere length but is independent of titin and nebulin.Journal of molecular and cellular cardiology · 2016Article
- Nebulin, a multi-functional giant.The Journal of experimental biology · 2016Review
- Tropomodulin 1 directly controls thin filament length in both wild-type and tropomodulin 4-deficient skeletal muscle.Development (Cambridge, England) · 2015Article
- How Cells Measure Length on Subcellular Scales.Trends in cell biology · 2015Review
- Intracellular Scaling Mechanisms.Cold Spring Harbor perspectives in biology · 2015Review
- Nebulette knockout mice have normal cardiac function, but show Z-line widening and up-regulation of cardiac stress markers.Cardiovascular research · 2015Article
- Subcellular size.Cold Spring Harbor perspectives in biology · 2015Review
- Effect of levosimendan on the contractility of muscle fibers from nemaline myopathy patients with mutations in the nebulin gene.Skeletal muscle · 2015Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 3 institutions in 1 country.
Funding
Abstract
To generate force, striated muscle requires overlap between uniform-length actin and myosin filaments. The hypothesis that a nebulin ruler mechanism specifies thin filament lengths by targeting where tropomodulin (Tmod) caps the slow-growing, pointed end has not been rigorously tested. Using fluorescent microscopy and quantitative image analysis, we found that nebulin extended 1.01-1.03 mum from the Z-line, but Tmod localized 1.13-1.31 mum from the Z-line, in seven different rabbit skeletal muscles. Because nebulin does not extend to the thin filament pointed ends, it can neither target Tmod capping nor specify thin filament lengths. We found instead a strong correspondence between thin filament lengths and titin isoform sizes for each muscle. Our results suggest the existence of a mechanism whereby nebulin specifies the minimum thin filament length and sarcomere length regulates and coordinates pointed-end dynamics to maintain the relative overlap of the thin and thick filaments during myofibril assembly.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.