ArticleBiophysical journal2009
Fusion step-specific influence of cholesterol on SNARE-mediated membrane fusion.
Article in Biophysical journal, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 36 citations in OpenAlex.
- Energetics of stalk intermediates elucidated from hydrostatic pressure effects on membrane fusion.bioRxiv : the preprint server for biology · 2026Article
- Invagination of Giant Unilamellar Vesicles upon Membrane Mixing with Native Vesicles.ACS omega · 2024Article
- Statins suppress cell-to-cell propagation of α-synuclein by lowering cholesterol.Cell death & disease · 2023Article
- Review
- Cholesterol stabilizes recombinant exocytic fusion pores by altering membrane bending rigidity.Biophysical journal · 2021Article
- Membrane cholesterol mediates the cellular effects of monolayer graphene substrates.Nature communications · 2018Article
- Revisit the Correlation between the Elastic Mechanics and Fusion of Lipid Membranes.Scientific reports · 2016Article
- High cholesterol obviates a prolonged hemifusion intermediate in fast SNARE-mediated membrane fusion.Biophysical journal · 2015Article
- Lipid molecules influence early stages of yeast SNARE-mediated membrane fusion.Physical biology · 2015Article
- ACAT1/SOAT1 as a therapeutic target for Alzheimer's disease.Future medicinal chemistry · 2015Review
- Niemann-Pick C disease and mobilization of lysosomal cholesterol by cyclodextrin.Journal of lipid research · 2014Review
- Characterisation of Weibel-Palade body fusion by amperometry in endothelial cells reveals fusion pore dynamics and the effect of cholesterol on exocytosis.Journal of cell science · 2013Article
- The lipid composition and physical properties of the yeast vacuole affect the hemifusion-fusion transition.Traffic (Copenhagen, Denmark) · 2013Article
- Islet cholesterol accumulation due to loss of ABCA1 leads to impaired exocytosis of insulin granules.Diabetes · 2011Article
- Changes in brain cholesterol metabolome after excitotoxicity.Molecular neurobiology · 2010Review
- Structure of sterol aliphatic chains affects yeast cell shape and cell fusion during mating.Proceedings of the National Academy of Sciences of the United States of America · 2010Article
- Complex lipid requirements for SNARE- and SNARE chaperone-dependent membrane fusion.The Journal of biological chemistry · 2009Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Cholesterol is a major component of biological membranes and is known to affect vesicle fusion. However, the mechanism by which cholesterol modulates SNARE-dependent intracellular fusion is not well understood. Using the fluorescence assay and dye-labeled SNAREs and the fluorescent lipids, we dissected cholesterol effects on individual fusion steps including SNARE complex formation, hemifusion, pore formation, and pore dilation. At physiological high concentrations, cholesterol stimulated hemifusion as much as 30-fold, but its stimulatory effect diminished to 10-fold and three-fold for subsequent pore formation and pore expansion at 40 mol %, respectively. The results show that cholesterol serves as a strong stimulator for hemifusion but acts as mild stimulators for pore opening and expansion. Strong stimulation of hemifusion and mild stimulation of pore formation are consistent with the fusion model based on the intrinsic negative curvature of cholesterol. However, even a milder effect of cholesterol on pore expansion is contradictory to such a simple curvature-based prediction. Thus, we speculate that cholesterol also affects the conformation of the transmembrane domains of SNAREs, which modulates the fusion kinetics.
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