Evidence map›Paper›PMID 19253965›Full record

ArticleLangmuir : the ACS journal of surfaces and colloids2009

Fabrication of DNA microarrays with poly(L-glutamic acid) monolayers on gold substrates for SPR imaging measurements.

Yulin Chen, Anh Nguyen, Lifang Niu, Robert M Corn

Abstract read
In one paragraph

Article in Langmuir : the ACS journal of surfaces and colloids, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.

0numbers the graph read from it
0cells of the map it votes in
19citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

19 citing papers in PubMed.

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  6. Silica nanowire arrays for diffraction-based bioaffinity sensing.Chemistry (Weinheim an der Bergstrasse, Germany) · 2014
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Yulin ChenDepartment of Chemistry, University of California--Irvine, Irvine, California 92697, USA.
Anh Nguyen
Lifang Niu
Robert M Corn

Funding

SPR IMAGING STUDIES OF PROTEIN/DNA INTERACTIONSR01GM059622 · NIGMS · UNIVERSITY OF WISCONSIN MADISON · PI CORN, ROBERT M · 1999 to 2018
$5.1M
Development of SPR/MS protein array platformR21RR018475 · NCRR · INTRINSIC BIOPROBES, INC. · PI NEDELKOV, DOBRIN · 2005 to 2005
$147k
NCRR NIH HHS 1R21 RR018475-01A2NCRR NIH HHS R21 RR018475NIGMS NIH HHS 2R01 GM059622-04NIGMS NIH HHS R01 GM059622
6 · The paper itself

Abstract

Robust single-stranded DNA (ssDNA) microarrays are created by attaching amine-modified oligonucleotides to a monolayer of poly(L-glutamic acid) (pGlu) that is electrostatically adsorbed onto a chemically modified gold thin film. This surface attachment chemistry methodology is first characterized with a combination of polarization-modulation Fourier transform infrared (PM-FTIR) spectroscopy and surface plasmon resonance (SPR) angle shift measurements. SPR imaging (SPRI) measurements of these ssDNA microarrays are then used to study two surface bioaffinity interactions: (i) the quantitative hybridization adsorption of complementary ssDNA onto mixed ssDNA microarray elements and (ii) the adsorption of single-stranded binding protein (SSB) onto fully and partially hybridized DNA microarray elements. The Langmuir adsorption coefficient (K(Ads)) of SSB binding to ssDNA was determined to be (5.5 +/- 0.4) x 10(9) M(-1).

Indexed as

AdsorptionGoldMolecular StructureOligonucleotide Array Sequence AnalysisPolyglutamic AcidSpectroscopy, Fourier Transform InfraredSurface Plasmon ResonanceSurface PropertiesGoldPolyglutamic Acid

Identifiers

PMID19253965
PMCPMC2726778

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.