ArticleEukaryotic cell2009
Yap1-regulated glutathione redox system curtails accumulation of formaldehyde and reactive oxygen species in methanol metabolism of Pichia pastoris.
Article in Eukaryotic cell, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 55 citations in OpenAlex.
- Systematic optimization ofEngineering microbiology · 2026Review
- Yeast Midas' touch: recent advances in the valorisation of methanol by yeasts.World journal of microbiology & biotechnology · 2025Review
- Balancing Doses of EL222 and Light Improves Optogenetic Induction of Protein Production in Komagataella phaffii.Biotechnology and bioengineering · 2025Article
- Design and construction of light-regulated gene transcription and protein translation systems in yeast P. Pastoris.Journal of advanced research · 2025Article
- Methylotrophic yeastFrontiers in microbiology · 2025Article
- Role of Dissimilative Pathway ofMicroorganisms · 2022Article
- Comparative transcriptome and metabolome analyses reveal the methanol dissimilation pathway of Pichia pastoris.BMC genomics · 2022Article
- Advances in Cell Engineering of theMetabolites · 2022Review
- Metabolic regulation adapting to high methanol environment in the methylotrophic yeast Ogataea methanolica.Microbial biotechnology · 2021Article
- Biomarkers allow detection of nutrient limitations and respective supplementation for elimination in Pichia pastoris fed-batch cultures.Microbial cell factories · 2017Article
- Insights into the prevalence and underlying causes of clonal variation through transcriptomic analysis in Pichia pastoris.Applied microbiology and biotechnology · 2017Article
- The mechanism of a formaldehyde-sensing transcriptional regulator.Scientific reports · 2016Article
- Molecular optimization of rabies virus glycoprotein expression in Pichia pastoris.Microbial biotechnology · 2016Article
- Overexpression of the transcription factor Yap1 modifies intracellular redox conditions and enhances recombinant protein secretion.Microbial cell (Graz, Austria) · 2014Article
- Defects in GABA metabolism affect selective autophagy pathways and are alleviated by mTOR inhibition.EMBO molecular medicine · 2014Article
- Biomarkers to evaluate the effects of temperature and methanol on recombinant Pichia pastoris.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2014Article
- Peroxisomal catalase deficiency modulates yeast lifespan depending on growth conditions.Aging · 2013Article
- Physiological response of Pichia pastoris GS115 to methanol-induced high level production of the Hepatitis B surface antigen: catabolic adaptation, stress responses, and autophagic processes.Microbial cell factories · 2012Article
- Expression of the laccase gene from a white rot fungus in Pichia pastoris can enhance the resistance of this yeast to H2O2-mediated oxidative stress by stimulating the glutathione-based antioxidative system.Applied and environmental microbiology · 2012Article
- Glutathione homeostasis and functions: potential targets for medical interventions.Journal of amino acids · 2012Article
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The glutathione redox system, including the glutathione biosynthesis and glutathione regeneration reaction, has been found to play a critical role in the yeast Pichia pastoris during growth on methanol, and this regulation was at least partly executed by the transcription factor PpYap1. During adaptation to methanol medium, PpYap1 transiently localized to the nucleus and activated the expression of the glutathione redox system and upregulated glutathione reductase 1 (Glr1). Glr1 activates the regeneration of the reduced form of glutathione (GSH). Depletion of Glr1 caused a severe growth defect on methanol and hypersensitivity to formaldehyde (HCHO), which could be complemented by addition of GSH to the medium. Disruption of the genes for the HCHO-oxidizing enzymes PpFld1 and PpFgh1 caused a comparable phenotype, but disruption of the downstream gene PpFDH1 did not, demonstrating the importance of maintaining intracellular GSH levels. Absence of the peroxisomal glutathione peroxidase Pmp20 also triggered nuclear localization of PpYap1, and although cells were not sensitive to HCHO, growth on methanol was again severely impaired due to oxidative stress. Thus, the PpYap1-regulated glutathione redox system has two important roles, i.e., HCHO metabolism and detoxification of reactive oxygen species.
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