ArticleThe Plant cell2009
The RPN5 subunit of the 26s proteasome is essential for gametogenesis, sporophyte development, and complex assembly in Arabidopsis.
Article in The Plant cell, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 40 papers.
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Who cites it
40 citing papers in PubMed, 81 citations in OpenAlex.
- Fumarylacetoacetate hydrolase targeted by a Fusarium graminearum effector positively regulates wheat FHB resistance.Nature communications · 2025Article
- Genetic variation in ZmKW1 contributes to kernel weight and size in dent corn and popcorn.Plant biotechnology journal · 2024Article
- Contrasting Impacts of Ubiquitin Overexpression on Arabidopsis Growth and Development.Plants (Basel, Switzerland) · 2024Article
- Proteasome Dysfunction Leads to Suppression of the Hypoxic Response Pathway in Arabidopsis.International journal of molecular sciences · 2022Article
- The RPN12a proteasome subunit is essential for the multiple hormonal homeostasis controlling the progression of leaf senescence.Communications biology · 2022Article
- The ubiquitin-26S proteasome system and autophagy relay proteome homeostasis regulation during silique development.The Plant journal : for cell and molecular biology · 2022Article
- Transcriptome and association mapping revealed functional genes respond to drought stress inFrontiers in plant science · 2022Article
- Genome-Wide Association Studies for Sex Determination and Cross-Compatibility in Water Yam (Plants (Basel, Switzerland) · 2021Article
- Leaf senescence: progression, regulation, and application.Molecular horticulture · 2021Review
- Multiple Layers of Regulation on Leaf Senescence: New Advances and Perspectives.Frontiers in plant science · 2021Review
- Advances on Plant Ubiquitylome-From Mechanism to Application.International journal of molecular sciences · 2020Review
- iTRAQ-based proteomic analysis reveals several key metabolic pathways associated with male sterility inRSC advances · 2020Article
- The Role and Regulation of Autophagy and the Proteasome During Aging and Senescence in Plants.Genes · 2019Review
- Genome-wide identification and expression analysis of the E2 gene family in potato.Molecular biology reports · 2019Article
- Resequencing 200 Flax Cultivated Accessions Identifies Candidate Genes Related to Seed Size and Weight and Reveals Signatures of Artificial Selection.Frontiers in plant science · 2019Article
- Proteasomal degradation of BRAHMA promotes Boron tolerance in Arabidopsis.Nature communications · 2018Article
- iTRAQ-Based Proteomics Analyses of Sterile/Fertile Anthers from a Thermo-Sensitive Cytoplasmic Male-Sterile Wheat withInternational journal of molecular sciences · 2018Article
- Proteasome storage granules protect proteasomes from autophagic degradation upon carbon starvation.eLife · 2018Article
- Suitability of two distinct approaches for the high-throughput study of the post-embryonic effects of embryo-lethal mutations in Arabidopsis.Scientific reports · 2017Article
- De Novo Disruption of the Proteasome Regulatory Subunit PSMD12 Causes a Syndromic Neurodevelopmental Disorder.American journal of human genetics · 2017Article
Corrections and comments
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Authors and funding
11 authors at 3 institutions in 1 country.
Funding
Abstract
The 26S proteasome is an essential multicatalytic protease complex that degrades a wide range of intracellular proteins, especially those modified with ubiquitin. Arabidopsis thaliana and other plants use pairs of genes to encode most of the core subunits, with both of the isoforms often incorporated into the mature complex. Here, we show that the gene pair encoding the regulatory particle non-ATPase subunit (RPN5) has a unique role in proteasome function and Arabidopsis development. Homozygous rpn5a rpn5b mutants could not be generated due to a defect in male gametogenesis. While single rpn5b mutants appear wild-type, single rpn5a mutants display a host of morphogenic defects, including abnormal embryogenesis, partially deetiolated development in the dark, a severely dwarfed phenotype when grown in the light, and infertility. Proteasome complexes missing RPN5a are less stable in vitro, suggesting that some of the rpn5a defects are caused by altered complex integrity. The rpn5a phenotype could be rescued by expression of either RPN5a or RPN5b, indicating functional redundancy. However, abnormal phenotypes generated by overexpression implied that paralog-specific functions also exist. Collectively, the data point to a specific role for RPN5 in the plant 26S proteasome and suggest that its two paralogous genes in Arabidopsis have both redundant and unique roles in development.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.