ArticleJournal of bacteriology2009
A vitamin B12-based system for conditional expression reveals dksA to be an essential gene in Myxococcus xanthus.
Article in Journal of bacteriology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 34 citations in OpenAlex.
- PexR is a noncanonical regulator of the peroxide stress response in bacteria.Nucleic acids research · 2025Article
- Article
- House of CarDs: Functional insights into the transcriptional regulator CdnL.Molecular microbiology · 2024Review
- Role of the CarH photoreceptor protein environment in the modulation of cobalamin photochemistry.Biophysical journal · 2021Article
- Light-Triggered Carotenogenesis inMicroorganisms · 2021Review
- CdbA is a DNA-binding protein and c-di-GMP receptor important for nucleoid organization and segregation in Myxococcus xanthus.Nature communications · 2020Article
- Plasticity in oligomerization, operator architecture, and DNA binding in the mode of action of a bacterial BThe Journal of biological chemistry · 2018Article
- CRISPR/dCas9-mediated transcriptional improvement of the biosynthetic gene cluster for the epothilone production in Myxococcus xanthus.Microbial cell factories · 2018Article
- A New Facet of Vitamin BAnnual review of biochemistry · 2017Review
- Structural insights into RNA polymerase recognition and essential function of Myxococcus xanthus CdnL.PloS one · 2014Article
- Two systems for conditional gene expression in Myxococcus xanthus inducible by isopropyl-β-D-thiogalactopyranoside or vanillate.Journal of bacteriology · 2012Article
- Comprehensive set of integrative plasmid vectors for copper-inducible gene expression in Myxococcus xanthus.Applied and environmental microbiology · 2012Article
- The anaerobe-specific orange protein complex of Desulfovibrio vulgaris hildenborough is encoded by two divergent operons coregulated by σ54 and a cognate transcriptional regulator.Journal of bacteriology · 2011Article
- Employing site-specific recombination for conditional genetic analysis in Sinorhizobium meliloti.Applied and environmental microbiology · 2011Article
- Genetic components of stringent response in Vibrio cholerae.The Indian journal of medical research · 2011Review
- CdnL, a member of the large CarD-like family of bacterial proteins, is vital for Myxococcus xanthus and differs functionally from the global transcriptional regulator CarD.Nucleic acids research · 2010Article
- Genes involved in yellow pigmentation of Cronobacter sakazakii ES5 and influence of pigmentation on persistence and growth under environmental stress.Applied and environmental microbiology · 2010Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Myxococcus xanthus is a prokaryotic model system for the study of multicellular development and the response to blue light. The previous analyses of these processes and the characterization of new genes would benefit from a robust system for controlled gene expression, which has been elusive so far for this bacterium. Here, we describe a system for conditional expression of genes in M. xanthus based on our recent finding that vitamin B12 and CarH, a MerR-type transcriptional repressor, together downregulate a photoinducible promoter. Using this system, we confirmed that M. xanthus rpoN, encoding sigma(54), is an essential gene, as reported earlier. We then tested it with ftsZ and dksA. In most bacteria, ftsZ is vital due to its role in cell division, whereas null mutants of dksA, whose product regulates the stringent response via transcriptional control of rRNA and amino acid biosynthesis promoters, are viable but cause pleiotropic effects. As with rpoN, it was impossible to delete endogenous ftsZ or dksA in M. xanthus except in a merodiploid background carrying another functional copy, which indicates that these are essential genes. B12-based conditional expression of ftsZ was insufficient to provide the high intracellular FtsZ levels required. With dksA, as with rpoN, cells were viable under permissive but not restrictive conditions, and depletion of DksA or sigma(54) produced filamentous, aberrantly dividing cells. dksA thus joins rpoN in a growing list of genes dispensable in many bacteria but essential in M. xanthus.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.