ArticleJournal of bacteriology2009
Pyruvate kinase-deficient Escherichia coli exhibits increased plasmid copy number and cyclic AMP levels.
Article in Journal of bacteriology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 54 citations in OpenAlex.
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- Sulfate limitation increases specific plasmid DNA yield and productivity in E. coli fed-batch processes.Microbial cell factories · 2023Article
- L-Alanine Prototrophic Suppressors Emerge from L-Alanine Auxotroph through Stress-Induced Mutagenesis inMicroorganisms · 2021Article
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- Application of Plasmid Engineering to Enhance Yield and Quality of Plasmid for Vaccine and Gene Therapy.Bioengineering (Basel, Switzerland) · 2019Article
- Elucidating the potential of crude cell extracts for producing pyruvate from glucose.Synthetic biology (Oxford, England) · 2018Article
- Effect of plasmid replication deregulation via inc mutations on E. coli proteome & simple flux model analysis.Microbial cell factories · 2015Article
- High-Level Production of Plasmid DNA by Escherichia coli DH5α ΩsacB by Introducing inc Mutations.Applied and environmental microbiology · 2014Article
- Constitutive expression of selected genes from the pentose phosphate and aromatic pathways increases the shikimic acid yield in high-glucose batch cultures of an Escherichia coli strain lacking PTS and pykF.Microbial cell factories · 2013Article
- Importance of understanding the main metabolic regulation in response to the specific pathway mutation for metabolic engineering of Escherichia coli.Computational and structural biotechnology journal · 2012Review
- Catabolic regulation analysis of Escherichia coli and its crp, mlc, mgsA, pgi and ptsG mutants.Microbial cell factories · 2011Article
- Identification of pyruvate kinase in methicillin-resistant Staphylococcus aureus as a novel antimicrobial drug target.Antimicrobial agents and chemotherapy · 2011Article
- Metabolic engineering for the production of shikimic acid in an evolved Escherichia coli strain lacking the phosphoenolpyruvate: carbohydrate phosphotransferase system.Microbial cell factories · 2010Article
- Factors affecting plasmid production in Escherichia coli from a resource allocation standpoint.Microbial cell factories · 2009Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
Abstract
Previously established consequences of abolishing pyruvate kinase (Pyk) activity in Escherichia coli during aerobic growth on glucose include reduced acetate production, elevated hexose monophosphate (HMP) pathway flux, elevated phosphoenolpyruvate carboxylase (Ppc) flux, and an increased ratio of phosphoenolpyruvate (PEP) to pyruvate. These traits inspired two hypotheses. First, the mutant (PB25) may maintain more plasmid than the wild type (JM101) by combining traits reported to facilitate plasmid DNA synthesis (i.e., decreased Pyk flux and increased HMP pathway and Ppc fluxes). Second, PB25 likely possesses a higher level of cyclic AMP (cAMP) than JM101. This is based on reports that connect elevated PEP/pyruvate ratios to phosphotransferase system signaling and adenylate cyclase activation. To test the first hypothesis, the strains were transformed with a pUC-based, high-copy-number plasmid (pGFPuv), and copy numbers were measured. PB25 exhibited a fourfold-higher copy number than JM101 when grown at 37 degrees C. At 42 degrees C, its plasmid content was ninefold higher than JM101 at 37 degrees C. To test the second hypothesis, cAMP was measured, and the results confirmed it to be higher in PB25 than JM101. This elevation was not enough to elicit a strong regulatory effect, however, as indicated by the comparative expression of the pGFPuv-based reporter gene, gfp(uv), under the control of the cAMP-responsive lac promoter. The elevated cAMP in PB25 suggests that Pyk may participate in glucose catabolite repression by serving among all of the factors that tighten gene expression.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.