ArticleJournal of bacteriology2009
The putative coupling protein TcpA interacts with other pCW3-encoded proteins to form an essential part of the conjugation complex.
Article in Journal of bacteriology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 41 citations in OpenAlex.
- Biology and engineering of integrative and conjugative elements: Construction and analyses of hybrid ICEs reveal element functions that affect species-specific efficiencies.PLoS genetics · 2022Article
- Pathogenicity and virulence ofVirulence · 2021Article
- Comparative Genomic Analysis Confirms Five Genetic Populations of the Select Agent,Microorganisms · 2020Article
- Type IV Coupling Proteins as Potential Targets to Control the Dissemination of Antibiotic Resistance.Frontiers in molecular biosciences · 2020Review
- Virulence Plasmids of the Pathogenic Clostridia.Microbiology spectrum · 2019Article
- Crystal structure of TcpK in complex with oriT DNA of the antibiotic resistance plasmid pCW3.Nature communications · 2018Article
- Review
- Critical Components of the Conjugation Machinery of the Integrative and Conjugative Element ICEBs1 of Bacillus subtilis.Journal of bacteriology · 2015Article
- Clostridium perfringens type A-E toxin plasmids.Research in microbiology · 2015Review
- Two novel membrane proteins, TcpD and TcpE, are essential for conjugative transfer of pCW3 in Clostridium perfringens.Journal of bacteriology · 2015Article
- Virulence Plasmids of Spore-Forming Bacteria.Microbiology spectrum · 2014Review
- Key components of the eight classes of type IV secretion systems involved in bacterial conjugation or protein secretion.Nucleic acids research · 2014Article
- Review
- Toxin plasmids of Clostridium perfringens.Microbiology and molecular biology reviews : MMBR · 2013Review
- Evolution of conjugation and type IV secretion systems.Molecular biology and evolution · 2013Article
- The 2.5 Å structure of the enterococcus conjugation protein TraM resembles VirB8 type IV secretion proteins.The Journal of biological chemistry · 2013Article
- Enterococcus faecalis PrgJ, a VirB4-like ATPase, mediates pCF10 conjugative transfer through substrate binding.Journal of bacteriology · 2012Article
- Article
- Article
- Type IV secretion in the obligatory intracellular bacterium Anaplasma phagocytophilum.Cellular microbiology · 2010Review
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
Abstract
Conjugative plasmids encode antibiotic resistance determinants or toxin genes in the anaerobic pathogen Clostridium perfringens. The paradigm conjugative plasmid in this bacterium is pCW3, a 47-kb tetracycline resistance plasmid that encodes the unique tcp transfer locus. The tcp locus consists of 11 genes, intP and tcpA-tcpJ, at least three of which, tcpA, tcpF, and tcpH, are essential for the conjugative transfer of pCW3. In this study we examined protein-protein interactions involving TcpA, the putative coupling protein. Use of a bacterial two-hybrid system identified interactions between TcpA and TcpC, TcpG, and TcpH. This analysis also demonstrated TcpA, TcpC, and TcpG self-interactions, which were confirmed by chemical cross-linking studies. Examination of a series of deletion and site-directed derivatives of TcpA identified the domains and motifs required for these interactions. Based on these results, we have constructed a model for this unique conjugative transfer apparatus.
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