ArticleBiochemical and biophysical research communications2009
Sphingosine 1-phosphate lyase enzyme assay using a BODIPY-labeled substrate.
Article in Biochemical and biophysical research communications, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 35 citations in OpenAlex.
- BODIPY-Labeled Estrogens for Fluorescence Analysis of Environmental Microbial Degradation.ACS omega · 2022Article
- Methuosis Contributes to Jaspine-B-Induced Cell Death.International journal of molecular sciences · 2022Article
- Fluorescence Anisotropy Studies on Bodipy (Pyrromethene 546) Dye as a Novel Thermal Probe.Journal of fluorescence · 2022Article
- Characterization of homologous sphingosine-1-phosphate lyase isoforms in the bacterial pathogen Burkholderia pseudomallei.Journal of lipid research · 2017Article
- A facile stable-isotope dilution method for determination of sphingosine phosphate lyase activity.Chemistry and physics of lipids · 2016Article
- LAPTM4B facilitates late endosomal ceramide export to control cell death pathways.Nature chemical biology · 2015Article
- Synthesis, spectroscopic, and in vitro investigations of 2,6-diiodo-BODIPYs with PDT and bioimaging applications.Journal of photochemistry and photobiology. B, Biology · 2015Article
- Sphingosine-1-phosphate lyase downregulation promotes colon carcinogenesis through STAT3-activated microRNAs.The Journal of clinical investigation · 2014Article
- RP-HPLC-fluorescence analysis of aliphatic aldehydes: application to aldehyde-generating enzymes HACL1 and SGPL1.Journal of lipid research · 2014Article
- Sphingosine phosphate lyase regulates myogenic differentiation via S1P receptor-mediated effects on myogenic microRNA expression.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2014Article
- Alkyne lipids as substrates for click chemistry-based in vitro enzymatic assays.Journal of lipid research · 2013Article
- Article
- Characterization of sphingosine-1-phosphate lyase activity by electrospray ionization-liquid chromatography/tandem mass spectrometry quantitation of (2E)-hexadecenal.Analytical biochemistry · 2011Article
- Sphingosine 1-phosphate lyase, a key regulator of sphingosine 1-phosphate signaling and function.Advances in enzyme regulation · 2010Review
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
Abstract
Sphingosine 1-phosphate lyase (SPL) is responsible for the irreversible catabolism of sphingosine 1-phosphate, which signals through five membrane receptors to mediate cell stress responses, angiogenesis, and lymphocyte trafficking. The standard assay for SPL activity utilizes a radioactive dihydrosphingosine 1-phosphate substrate and is expensive and cumbersome. In this study, we describe an SPL assay that employs an omega-labeled BODIPY-sphingosine 1-phosphate substrate, allowing fluorescent product detection by HPLC and incorporating advantages of the BODIPY fluorophore. The major aldehyde product is confirmed by reaction with 2,4-dinitrophenylhydrazine. The SPL-catalyzed reaction is linear over a 30 min time period and yields a K(m) of 35 microM for BODIPY-sphingosine 1-phosphate.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.