ArticleAnalytical biochemistry2009
Preparation of heteroduplex enhanced green fluorescent protein plasmid for in vivo mismatch repair activity assay.
Article in Analytical biochemistry, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 21 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
21 citing papers in PubMed.
- Article
- DNA repair and anti-cancer mechanisms in the long-lived bowhead whale.bioRxiv : the preprint server for biology · 2024Article
- MRE11A: a novel negative regulator of human DNA mismatch repair.Cellular & molecular biology letters · 2024Article
- Article
- FANCD2-Associated Nuclease 1 Partially Compensates for the Lack of Exonuclease 1 in Mismatch Repair.Molecular and cellular biology · 2021Article
- DNA Damage and Repair Deficiency in ALS/FTD-Associated Neurodegeneration: From Molecular Mechanisms to Therapeutic Implication.Frontiers in molecular neuroscience · 2021Review
- Fluorescent Probes of DNA Repair.ACS chemical biology · 2018Review
- Helraiser intermediates provide insight into the mechanism of eukaryotic replicative transposition.Nature communications · 2018Article
- The CREB Coactivator CRTC2 Is a Lymphoma Tumor Suppressor that Preserves Genome Integrity through Transcription of DNA Mismatch Repair Genes.Cell reports · 2015Article
- Article
- Human pluripotent stem cells have a novel mismatch repair-dependent damage response.The Journal of biological chemistry · 2014Article
- A plasmid-based lacZα gene assay for DNA polymerase fidelity measurement.Analytical biochemistry · 2013Article
- Maintenance of genomic stability in mouse embryonic stem cells: relevance in aging and disease.International journal of molecular sciences · 2013Article
- The predicted truncation from a cancer-associated variant of the MSH2 initiation codon alters activity of the MSH2-MSH6 mismatch repair complex.Molecular carcinogenesis · 2012Article
- Determining the functional significance of mismatch repair gene missense variants using biochemical and cellular assays.Hereditary cancer in clinical practice · 2012Article
- DNA mismatch repair proteins are required for efficient herpes simplex virus 1 replication.Journal of virology · 2011Article
- Mechanisms maintaining genomic integrity in embryonic stem cells and induced pluripotent stem cells.Experimental biology and medicine (Maywood, N.J.) · 2011Review
- In vitro studies of DNA mismatch repair proteins.Analytical biochemistry · 2011Article
- Article
- Natural and engineered nicking endonucleases--from cleavage mechanism to engineering of strand-specificity.Nucleic acids research · 2011Review
Corrections and comments
- Erratum issued
Authors and funding
6 authors.
Funding
Abstract
Preparation of heteroduplexes in large quantities with high purity is essential for the measurement of DNA mismatch repair (MMR) activity. Here we report a rapid, less labor-intensive method for the preparation of a heteroduplex plasmid that expresses the enhanced green fluorescent protein (EGFP) if the mismatch is repaired correctly. The method involves the use of a wild-type and a mutated EGFP expression plasmid and a few steps of enzymatic digestion. When the constructed heteroduplex EGFP plasmid was transfected into MMR-proficient and -deficient cell lines, the number of EGFP-expressing cells was much higher in the MMR-proficient cells than in the MMR-deficient cells, suggesting that the heteroduplex can be used for MMR activity assay in live model systems.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.