ArticleIn vitro cellular & developmental biology. Animal
Primary culture of insect midgut cells.
Article in In vitro cellular & developmental biology. Animal. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 10 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
10 citing papers in PubMed.
- Link between Energy Investment in Biosynthesis and Proteostasis: Testing the Cost-Quality Hypothesis in Insects.Insects · 2023Article
- Article
- Isolation, establishment and characterization of new insect cell line derived from midgut of rice weevil, Sitophilus oryzae.In vitro cellular & developmental biology. Animal · 2020Article
- Establishment of two midgut cell lines from the fall armyworm, Spodoptera frugiperda (Lepidoptera: Noctuidae).In vitro cellular & developmental biology. Animal · 2020Article
- Distribution of Glycan Motifs at the Surface of Midgut Cells in the Cotton Leafworm (Frontiers in physiology · 2017Article
- Establishment and characterization of three embryonic cell lines of beet armyworm, Spodoptera exigua (Lepidoptera: Noctuidae).Cytotechnology · 2016Article
- Developmental Expression of Ecdysone-Related Genes Associated With Metamorphic Changes During Midgut Remodeling of Silkworm Bombyx mori (Lepidoptera:Bombycidae).Journal of insect science (Online) · 2016Article
- Establishment and characterization of a novel cell line from midgut tissue of Helicoverpa armigera (Lepidoptera: Noctuidae).In vitro cellular & developmental biology. Animal · 2015Article
- Article
- Establishing midgut cell culture from Rhynchophorus ferrugineus (Olivier) and toxicity assessment against ten different insecticides.In vitro cellular & developmental biology. Animal · 2014Article
Corrections and comments
- Erratum issued
Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
This protocol describes the preparation of primary cell cultures from Lepidopteran midgut. These cultures have been used to identify factors that control midgut growth and differentiation, cell responses to these factors, effects of toxins on midgut growth, and the regulation of cell physiology. The protocol is divided into (1) procedures for cell collection, (2) composition of the culture, and (3) assay methods used for cell health, proliferation, and differentiation. Collection and setup require 4-6 h. Once established, a culture can survive several months at 25 degrees C, be kept a year or longer at 4 degrees C, or be frozen for indefinite storage.
Indexed as
Identifiers
19247724What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.