ArticlePloS one2009
Temporally-controlled site-specific recombination in zebrafish.
Article in PloS one, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 84 papers.
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Who cites it
84 citing papers in PubMed, 206 citations in OpenAlex.
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- Heterogeneity and genomic loci of ubiquitous transgenic Cre reporter lines in zebrafish.Developmental dynamics : an official publication of the American Association of Anatomists · 2022Article
- Endothelial versus pronephron fate decision is modulated by the transcription factors Cloche/Npas4l, Tal1, and Lmo2.Science advances · 2022Article
- The use of transgenics in the laboratory axolotl.Developmental dynamics : an official publication of the American Association of Anatomists · 2022Review
- Inducible and tissue-specific cell labeling in Cre-ERDevelopment, growth & differentiation · 2022Article
- Wt1 transcription factor impairs cardiomyocyte specification and drives a phenotypic switch from myocardium to epicardium.Development (Cambridge, England) · 2022Article
- zMADM (zebrafish mosaic analysis with double markers) for single-cell gene knockout and dual-lineage tracing.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
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- Non-Rodent Genetic Animal Models for Studying Tauopathy: Review ofInternational journal of molecular sciences · 2021Review
- Visualizing the Itch-Sensing Skin Arbors.The Journal of investigative dermatology · 2021Article
- A robust and flexible CRISPR/Cas9-based system for neutrophil-specific gene inactivation in zebrafish.Journal of cell science · 2021Article
- Persistent Ventricle Partitioning in the Adult Zebrafish Heart.Journal of cardiovascular development and disease · 2021Article
- From Stripes to a Beating Heart: Early Cardiac Development in Zebrafish.Journal of cardiovascular development and disease · 2021Review
- Endogenous zebrafish proneural Cre drivers generated by CRISPR/Cas9 short homology directed targeted integration.Scientific reports · 2021Article
24 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Conventional use of the site-specific recombinase Cre is a powerful technology in mouse, but almost absent in other vertebrate model organisms. In zebrafish, Cre-mediated recombination efficiency was previously very low. Here we show that using transposon-mediated transgenesis, Cre is in fact highly efficient in this organism. Furthermore, temporal control of recombination can be achieved by using the ligand-inducible CreER(T2). Site-specific recombination only occurs upon administration of the drug tamoxifen (TAM) or its active metabolite, 4-hydroxy-tamoxifen (4-OHT). Cre-mediated recombination is detectable already 4 or 2 hours after administration of TAM or 4-OHT, demonstrating fast recombination kinetics. In addition, low doses of TAM allow mosaic labeling of single cells. Combined, our results show that conditional Cre/lox will be a valuable tool for both, embryonic and adult zebrafish studies. Furthermore, single copy insertion transgenesis of Cre/lox constructs suggest a strategy suitable also for other organisms.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.