ArticlePLoS pathogens2009
Molecular evolution of a viral non-coding sequence under the selective pressure of amiRNA-mediated silencing.
Article in PLoS pathogens, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 40 papers.
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Who cites it
40 citing papers in PubMed.
- Molecular Mechanisms Underlying Cucumber Resistance to Cucumber Green Mottle Mosaic Virus: From Host-Virus Interactions to Precision Breeding Strategies.International journal of molecular sciences · 2026Review
- amiR414e-mediated silencing confers resistance to Cotton leaf curl Multan virus in cotton.Molecular biology reports · 2026Article
- Research Progress on miRNAs and Artificial miRNAs in Insect and Disease Resistance and Breeding in Plants.Genes · 2024Review
- Innate immune pathways act synergistically to constrain RNA virus evolution in Drosophila melanogaster.Nature ecology & evolution · 2022Article
- Polycistronic Artificial microRNA-Mediated Resistance to Cucumber Green Mottle Mosaic Virus in Cucumber.International journal of molecular sciences · 2021Article
- Optimized RNA-Silencing Strategies forPlants (Basel, Switzerland) · 2021Article
- Identifying potential human and medicinal plant microRNAs against SARS-CoV-2 3'UTR region: A computational genomics assessment.Computers in biology and medicine · 2021Article
- Resistance to Turnip Mosaic Virus in the FamilyThe plant pathology journal · 2021Review
- Fine-tune control of targeted RNAi efficacy by plant artificial small RNAs.Nucleic acids research · 2020Article
- Artificial Small RNA-Based Silencing Tools for Antiviral Resistance in Plants.Plants (Basel, Switzerland) · 2020Review
- Detection of Plant Viruses and Disease Management: Relevance of Genetic Diversity and Evolution.Frontiers in plant science · 2020Review
- Multi-targeting of viral RNAs with synthetic trans-acting small interfering RNAs enhances plant antiviral resistance.The Plant journal : for cell and molecular biology · 2019Article
- Artificial microRNA-mediated resistance to cucumber green mottle mosaic virus in Nicotiana benthamiana.Planta · 2019Article
- PAmiRDB: A web resource for plant miRNAs targeting viruses.Scientific reports · 2019Article
- Nanotechnology based approaches for detection and delivery of microRNA in healthcare and crop protection.Journal of nanobiotechnology · 2018Review
- Plant Responses to Pathogen Attack: Small RNAs in Focus.International journal of molecular sciences · 2018Review
- The Search for Resistance to Cassava Mosaic Geminiviruses: How Much We Have Accomplished, and What Lies Ahead.Frontiers in plant science · 2017Review
- An Optimized Transient Dual Luciferase Assay for Quantifying MicroRNA Directed Repression of Targeted Sequences.Frontiers in plant science · 2017Article
- A putative antiviral role of plant cytidine deaminases.F1000Research · 2017Article
- MicroRNA-Mediated Gene Silencing in Plant Defense and Viral Counter-Defense.Frontiers in microbiology · 2017Review
Corrections and comments
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Authors and funding
8 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Plant microRNAs (miRNA) guide cleavage of target mRNAs by DICER-like proteins, thereby reducing mRNA abundance. Native precursor miRNAs can be redesigned to target RNAs of interest, and one application of such artificial microRNA (amiRNA) technology is to generate plants resistant to pathogenic viruses. Transgenic Arabidopsis plants expressing amiRNAs designed to target the genome of two unrelated viruses were resistant, in a highly specific manner, to the appropriate virus. Here, we pursued two different goals. First, we confirmed that the 21-nt target site of viral RNAs is both necessary and sufficient for resistance. Second, we studied the evolutionary stability of amiRNA-mediated resistance against a genetically plastic RNA virus, TuMV. To dissociate selective pressures acting upon protein function from those acting at the RNA level, we constructed a chimeric TuMV harboring a 21-nt, amiRNA target site in a non-essential region. In the first set of experiments designed to assess the likelihood of resistance breakdown, we explored the effect of single nucleotide mutation within the target 21-nt on the ability of mutant viruses to successfully infect amiRNA-expressing plants. We found non-equivalency of the target nucleotides, which can be divided into three categories depending on their impact in virus pathogenicity. In the second set of experiments, we investigated the evolution of the virus mutants in amiRNA-expressing plants. The most common outcome was the deletion of the target. However, when the 21-nt target was retained, viruses accumulated additional substitutions on it, further reducing the binding/cleavage ability of the amiRNA. The pattern of substitutions within the viral target was largely dominated by G to A and C to U transitions.
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