ArticleJournal of experimental botany2009
NtCP56, a new cysteine protease in Nicotiana tabacum L., involved in pollen grain development.
Article in Journal of experimental botany, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 33 citations in OpenAlex.
- PtCP1 Is an Extraplastidial Cysteine Protease Involved in Leaf Protein Degradation ofPlants (Basel, Switzerland) · 2026Article
- Regulation of Pollen Viability, Pollen Tube Growth and Seed Development in Maize by Application of Cysteine Protease ZmPCP.Plants (Basel, Switzerland) · 2026Article
- High-Quality Genome Assembly and Genome-Wide Association Study of Male Sterility Provide Resources for Flax Improvement.Plants (Basel, Switzerland) · 2023Article
- Modifying the Expression of Cysteine Protease GeneInternational journal of molecular sciences · 2023Article
- Genotyping by Sequencing for SNP-Based Linkage Analysis and the Development of KASPar Markers for Male Sterility and Polyembryony in Citrus.Plants (Basel, Switzerland) · 2023Article
- MYB2 Is Important for Tapetal PCD and Pollen Development by Directly Activating Protease Expression inInternational journal of molecular sciences · 2022Article
- How Cysteine Protease GeneInternational journal of molecular sciences · 2021Article
- PtomtAPX, a mitochondrial ascorbate peroxidase, plays an important role in maintaining the redox balance of Populus tomentosa Carr.Scientific reports · 2019Article
- Targeted expression of a cysteine protease (AdCP) in tapetum induces male sterility in Indian mustard, Brassica juncea.Functional & integrative genomics · 2019Article
- A proteomic study of cysteine protease induced cell death in anthers of male sterile tobacco transgenic plants.Physiology and molecular biology of plants : an international journal of functional plant biology · 2019Article
- Genome-Wide Identification of Papain-Like Cysteine Proteases inFrontiers in plant science · 2019Article
- Papain-like and legumain-like proteases in rice: genome-wide identification, comprehensive gene feature characterization and expression analysis.BMC plant biology · 2018Article
- Identification of miRNAs with potential roles in regulation of anther development and male-sterility in 7B-1 male-sterile tomato mutant.BMC genomics · 2015Article
- Identification, Characterization and Down-Regulation of Cysteine Protease Genes in Tobacco for Use in Recombinant Protein Production.PloS one · 2015Article
- Comprehensive analysis of cystatin family genes suggests their putative functions in sexual reproduction, embryogenesis, and seed formation.Journal of experimental botany · 2014Article
- The cysteine protease CEP1, a key executor involved in tapetal programmed cell death, regulates pollen development in Arabidopsis.The Plant cell · 2014Article
- Expression of a pathogen-induced cysteine protease (AdCP) in tapetum results in male sterility in transgenic tobacco.Functional & integrative genomics · 2014Article
- Silencing of the CaCP gene delays salt- and osmotic-induced leaf senescence in Capsicum annuum L.International journal of molecular sciences · 2014Article
- Identification of putative TAL effector targets of the citrus canker pathogens shows functional convergence underlying disease development and defense response.BMC genomics · 2014Article
- Exon skipping of AGAMOUS homolog PrseAG in developing double flowers of Prunus lannesiana (Rosaceae).Plant cell reports · 2013Article
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Proteinases play a critical role in developmental homeostasis and in response to environ-mental stimuli. Our present research reports that a new cysteine protease, NtCP56, is involved in the development of pollen grains in Nicotiana tabacum L. The NtCP56 gene, which encodes a protein of 361 amino acid residues with a calculated molecular mass of 40 kDa, is strongly expressed in anthers. The recombinant NtCP56 showed a high activity towards casein. Kinetic analysis revealed a K(m) of 2.20 mg ml(-1) and V(max) of 11.07 microg ml(-1) min(-1). The recombinant NtCP56 retained more than 50% of its maximum enzymatic activity from 20 degrees C to 60 degrees C with an optimum Tm range of 30-50 degrees C. The enzyme had a maximum activity at approximately pH 6.5. Suppression of the NtCP56 gene in anti-sense transgenic tobaccos resulted in the sterility of pollen grains. Our data indicated that, as a cysteine protease, NtCP56 might play an important role in pollen development.
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