ArticleNucleic acids research2009
Asymmetric DNA requirements in Xer recombination activation by FtsK.
Article in Nucleic acids research, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 26 citations in OpenAlex.
- Investigating the mechanism of rough phenotype in a naturally attenuatedFrontiers in medicine · 2024Article
- DNA Segregation in Enterobacteria.EcoSal Plus · 2023Review
- MukBEF-dependent chromosomal organization in widenedFrontiers in microbiology · 2023Article
- The Xer activation factor of TLCΦ expands the possibilities for Xer recombination.Nucleic acids research · 2022Article
- The TLCΦ satellite phage harbors a Xer recombination activation factor.Proceedings of the National Academy of Sciences of the United States of America · 2019Article
- Article
- TPM analyses reveal that FtsK contributes both to the assembly and the activation of the XerCD-dif recombination synapse.Nucleic acids research · 2014Article
- Topoisomerase I (TopA) is recruited to ParB complexes and is required for proper chromosome organization during Streptomyces coelicolor sporulation.Journal of bacteriology · 2013Article
- FtsK actively segregates sister chromosomes in Escherichia coli.Proceedings of the National Academy of Sciences of the United States of America · 2013Article
- Molecular motors for DNA translocation in prokaryotes.Current opinion in biotechnology · 2012Review
- Comprehensive prediction of chromosome dimer resolution sites in bacterial genomes.BMC genomics · 2011Article
- A defined terminal region of the E. coli chromosome shows late segregation and high FtsK activity.PloS one · 2011Article
- Are two better than one? Analysis of an FtsK/Xer recombination system that uses a single recombinase.Nucleic acids research · 2010Article
- Article
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Authors and funding
5 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In bacteria with circular chromosomes, homologous recombination events can lead to the formation of chromosome dimers. In Escherichia coli, chromosome dimers are resolved by the addition of a crossover by two tyrosine recombinases, XerC and XerD, at a specific site on the chromosome, dif. Recombination depends on a direct contact between XerD and a cell division protein, FtsK, which functions as a hexameric double stranded DNA translocase. Here, we have investigated how the structure and composition of DNA interferes with Xer recombination activation by FtsK. XerC and XerD each cleave a specific strand on dif, the top and bottom strand, respectively. We found that the integrity and nature of eight bottom-strand nucleotides and three top-strand nucleotides immediately adjacent to the XerD-binding site of dif are crucial for recombination. These nucleotides are probably not implicated in FtsK translocation since FtsK could translocate on single stranded DNA in both the 5'-3' and 3'-5' orientation along a few nucleotides. We propose that they are required to stabilize FtsK in the vicinity of dif for recombination to occur because the FtsK-XerD interaction is too transient or too weak in itself to allow for XerD catalysis.
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