ArticleFASEB journal : official publication of the Federation of American Societies for Experimental Biology2009
Amino acid-mediated heterotypic interaction governs performance of a hepatic tissue model.
Article in FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
19 citing papers in PubMed, 48 citations in OpenAlex.
- High-yield isolation of primary human hepatocytes from small liver samples.In vitro models · 2025Article
- Sacrificing Alginate in Decellularized Extracellular Matrix Scaffolds for Implantable Artificial Livers.Journal of functional biomaterials · 2025Article
- Bioengineering considerations in liver regenerative medicine.Journal of biological engineering · 2017Review
- Emerging In Vitro Liver Technologies for Drug Metabolism and Inter-Organ Interactions.Tissue engineering. Part B, Reviews · 2016Review
- Effect of Concentrated Fibroblast-Conditioned Media on In Vitro Maintenance of Rat Primary Hepatocyte.PloS one · 2016Article
- Long-term coculture strategies for primary hepatocytes and liver sinusoidal endothelial cells.Tissue engineering. Part C, Methods · 2015Article
- Microengineered cell and tissue systems for drug screening and toxicology applications: Evolution ofTechnology · 2015Article
- Dynamic interplay of flow and collagen stabilizes primary hepatocytes culture in a microfluidic platform.Lab on a chip · 2014Article
- Article
- Assembly of human organs from stem cells to study liver disease.The American journal of pathology · 2014Review
- Recent advances in 2D and 3D in vitro systems using primary hepatocytes, alternative hepatocyte sources and non-parenchymal liver cells and their use in investigating mechanisms of hepatotoxicity, cell signaling and ADME.Archives of toxicology · 2013Review
- Towards a three-dimensional microfluidic liver platform for predicting drug efficacy and toxicity in humans.Stem cell research & therapy · 2013Review
- Article
- Perspectives on whole-organ assembly: moving toward transplantation on demand.The Journal of clinical investigation · 2012Review
- Whole-organ tissue engineering: decellularization and recellularization of three-dimensional matrix scaffolds.Annual review of biomedical engineering · 2011Review
- Therapeutic potential of stem cell in liver regeneration.Frontiers of medicine · 2011Review
- Tissue-engineered model for real-time monitoring of liver inflammation.Tissue engineering. Part C, Methods · 2011Article
- Metabolic profiling based quantitative evaluation of hepatocellular metabolism in presence of adipocyte derived extracellular matrix.PloS one · 2011Article
- Pluripotent stem cell-derived hepatocyte-like cells.Biotechnology advancesReview
Corrections and comments
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Authors and funding
5 authors at 3 institutions in 1 country.
Funding
Abstract
Tissue-engineered models that mimic in vivo tissue organization offer the potential of capturing complex signaling pathways in vitro. In the liver, hepatocytes and endothelial cells are closely associated but separated by the extracellular matrix of the space of Disse. This unique configuration was mimicked by embedding primary hepatocytes in collagen gel and overlaying the matrix with endothelial cells. We demonstrate that during the first few days of culture, the secretion of albumin and fibrinogen was 2-fold higher in cocultures compared to hepatocytes alone. Hepatocyte function in both cultures stabilized to a similar level during the second week, suggesting that endothelial cells can induce the early recovery of hepatocytes after isolation and seeding. Endothelial cell-conditioned medium reproduced the effect of coculture in a dose-dependent fashion, suggesting a role for endothelial cell-derived soluble factors. Endothelial cell-conditioned medium increased mRNA levels of various acute-phase proteins such as albumin, fibrinogen, transferrin, and alpha-macroglobulin in hepatocytes. Surprisingly, the effect of endothelial cell-conditioned medium was not mediated by growth factors or cytokines, or by secreted extracellular matrix, but by the release of the amino acid proline, which mediates endogenous collagen synthesis by hepatocytes. These findings suggest an important role for proline secretion by endothelial cells as a paracrine factor regulating hepatocyte function.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.