ArticleThe Journal of biological chemistry2009
Covalently bound substrate at the regulatory site of yeast pyruvate decarboxylases triggers allosteric enzyme activation.
Article in The Journal of biological chemistry, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 46 citations in OpenAlex.
- Potent Inhibition ofACS infectious diseases · 2024Article
- Improving tyrosol production efficiency through shortening the allosteric signal transmission distance of pyruvate decarboxylase.Applied microbiology and biotechnology · 2023Article
- Allosteric inhibition ofPhilosophical transactions of the Royal Society of London. Series B, Biological sciences · 2023Article
- The Characterization of an Efficient Phenylpyruvate Decarboxylase KDC4427, Involved in 2-Phenylethanol and IAA Production from Bacterial Enterobacter sp. CGMCC 5087.Microbiology spectrum · 2022Article
- Enzymes for Efficient COThe protein journal · 2021Review
- Biochemical Characterization and Structural Insight into Interaction and Conformation Mechanisms ofMolecules (Basel, Switzerland) · 2021Article
- Allosteric regulation of menaquinone (vitamin KThe Journal of biological chemistry · 2020Article
- The Structure of an Oxalate Oxidoreductase Provides Insight into Microbial 2-Oxoacid Metabolism.Biochemistry · 2015Article
- Perturbation of the monomer-monomer interfaces of the benzoylformate decarboxylase tetramer.Biochemistry · 2014Article
- Computational evaluation of factors governing catalytic 2-keto acid decarboxylation.Journal of molecular modeling · 2014Article
- PolyprOnline: polyproline helix II and secondary structure assignment database.Database : the journal of biological databases and curation · 2014Article
- Analysis of gene expression and proteomic profiles of clonal genotypes from Theobroma cacao subjected to soil flooding.PloS one · 2014Article
- Decarboxylation of pyruvate to acetaldehyde for ethanol production by hyperthermophiles.Biomolecules · 2013Article
- A standard numbering scheme for thiamine diphosphate-dependent decarboxylases.BMC biochemistry · 2012Article
- Bifunctionality of the thiamin diphosphate cofactor: assignment of tautomeric/ionization states of the 4'-aminopyrimidine ring when various intermediates occupy the active sites during the catalysis of yeast pyruvate decarboxylase.Journal of the American Chemical Society · 2012Article
- Solid-state nuclear magnetic resonance studies delineate the role of the protein in activation of both aromatic rings of thiamin.Journal of the American Chemical Society · 2012Article
- Assignment of PolyProline II conformation and analysis of sequence--structure relationship.PloS one · 2011Article
- Structural insights into the prereaction state of pyruvate decarboxylase from Zymomonas mobilis .Biochemistry · 2010Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The mechanism by which the enzyme pyruvate decarboxylase from two yeast species is activated allosterically has been elucidated. A total of seven three-dimensional structures of the enzyme, of enzyme variants, or of enzyme complexes from two yeast species, three of them reported here for the first time, provide detailed atomic resolution snapshots along the activation coordinate. The prime event is the covalent binding of the substrate pyruvate to the side chain of cysteine 221, thus forming a thiohemiketal. This reaction causes the shift of a neighboring amino acid, which eventually leads to the rigidification of two otherwise flexible loops, one of which provides two histidine residues necessary to complete the enzymatically competent active site architecture. The structural data are complemented and supported by kinetic investigations and binding studies, providing a consistent picture of the structural changes occurring upon enzyme activation.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.