ArticleBJU international2009
Effect of hydrogen sulphide-donating sildenafil (ACS6) on erectile function and oxidative stress in rabbit isolated corpus cavernosum and in hypertensive rats.
Article in BJU international, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 37 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
37 citing papers in PubMed, 96 citations in OpenAlex.
- Detection of exhaled hydrogen sulphide gas in healthy human volunteers during intravenous administration of sodium sulphide.British journal of clinical pharmacology · 2010Trial
- The functions of hydrogen sulfide on the urogenital system of both males and females: from inception to the present.Naunyn-Schmiedeberg's archives of pharmacology · 2024Review
- Oxidative Stress and Erectile Dysfunction: Pathophysiology, Impacts, and Potential Treatments.Current issues in molecular biology · 2024Review
- Unveiling the Potential of Sulfur-Containing Gas Signaling Molecules in Acute Lung Injury: A Promising Therapeutic Avenue.Current issues in molecular biology · 2024Review
- Pills of Multi-Target HInternational journal of molecular sciences · 2024Review
- Supplementation of sperm cryopreservation media with HScientific reports · 2024Article
- The relationship between oxidative balance score and erectile dysfunction in the U.S. male adult population.Scientific reports · 2024Article
- Therapeutic Potential of Hydrogen Sulfide in Reproductive System Disorders.Biomolecules · 2024Review
- Role of hydrogen sulfide in the male reproductive system.Frontiers in endocrinology · 2024Review
- Hydrogen sulfide and its potential as a possible therapeutic agent in male reproduction.Frontiers in endocrinology · 2024Review
- Research Progress of HMolecules (Basel, Switzerland) · 2022Review
- Isolation, bioassay and 3D-QSAR analysis of 8-isopentenyl flavonoids from Epimedium sagittatum maxim. as PDE5A inhibitors.Chinese medicine · 2022Article
- Trends in HAntioxidants (Basel, Switzerland) · 2021Review
- Protective and therapeutic effects of sildenafil and tadalafil on aflatoxin B1-induced hepatocellular carcinoma.Molecular and cellular biochemistry · 2021Article
- Hydrogen Sulfide in Pharmacotherapy, Beyond the Hydrogen Sulfide-Donors.Biomolecules · 2020Review
- Article
- The dichotomous role of HBiochemical pharmacology · 2018Review
- International Union of Basic and Clinical Pharmacology. CII: Pharmacological Modulation of HPharmacological reviews · 2017Review
- Hydrogen sulfide, an enhancer of vascular nitric oxide signaling: mechanisms and implications.American journal of physiology. Cell physiology · 2017Review
- Long-term release of a thiobenzamide from a backbone functionalized poly(lactic acid).Polymer chemistry · 2015Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
9 authors at 4 institutions in 3 countries.
Funding
Abstract
OBJECTIVE To study the effect of the H(2)S-donating derivative of sildenafil (ACS6) compared to sildenafil citrate and sodium hydrosulphide (NaHS) on relaxation, superoxide formation and NADPH oxidase and type 5 phosphodiesterase (PDE5) expression in isolated rabbit cavernosal tissue and smooth muscle cells (CSMCs), and in vivo on indices of oxidative stress induced with buthionine sulphoximine (BSO). MATERIALS AND METHODS Relaxation was studied in an organ bath in response to carbachol and after incubation with interleukin-1beta for 12 h. CSMCs were incubated with tumour-necrosis factor-alpha or the thromboxane A(2) (TXA(2)) analogue, U46619, with or with no sildenafil citrate, ACS6 or NaHS for 16 h. Superoxide formation and the expression of p47(phox) (an active subunit of the NADPH oxidase complex) and PDE5 protein was then assessed using Western blotting. Rats were also treated with BSO (with or with no sildenafil citrate or ACS6) for 7 days; cavernosal cGMP, cAMP, glutathionine and plasma TXA(2) and 8-isoprostane F(2alpha) was measured by enzyme-linked immunosorbent assay. RESULTS ACS6 and sildenafil citrate relaxed cavernosal smooth muscle equipotently; NaHS alone had little effect at up to 100 microm. The formation of superoxide and expression of p47(phox) and PDE5 was reduced by ACS6, sildenafil citrate and NaHS (order of potency: ACS6 > sildenafil citrate > NaHS). The effects of ACS6 were blocked by inhibitors of protein kinase A (PKA) and PKG. In rats treated with BSO, both ASC6 and sildenafil citrate reduced the increased plasma levels of TXA(2) and 8-isoprostane F(2alpha) but increased cGMP, cAMP and glutathionine levels in corpus cavernosum. CONCLUSIONS By virtue of a dual action on PKA and PKG activation, ACS6 not only promotes erection, acutely, but might also have a long-term beneficial effect through inhibition of oxidative stress and downregulation of PDE5.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.