ArticleJournal of molecular biology2009
U30 of 7SK RNA forms a specific photo-cross-link with Hexim1 in the context of both a minimal RNA-binding site and a fully reconstituted 7SK/Hexim1/P-TEFb ribonucleoprotein complex.
Article in Journal of molecular biology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 22 citations in OpenAlex.
- Immunoprecipitation Methods to Isolate Messenger Ribonucleoprotein Complexes (mRNP).Advances in experimental medicine and biology · 2024Article
- Different views of the dynamic landscape covered by the 5'-hairpin of the 7SK small nuclear RNA.RNA (New York, N.Y.) · 2020Article
- Structural transitions in the RNA 7SK 5' hairpin and their effect on HEXIM binding.Nucleic acids research · 2020Article
- Carbodiimide reagents for the chemical probing of RNA structure in cells.RNA (New York, N.Y.) · 2019Article
- Hexim1, an RNA-controlled protein hub.Transcription · 2018Review
- The crystal structure of the 5΄ functional domain of the transcription riboregulator 7SK.Nucleic acids research · 2017Article
- Solution structure of the 5'-terminal hairpin of the 7SK small nuclear RNA.RNA (New York, N.Y.) · 2016Article
- An evolutionary conserved Hexim1 peptide binds to the Cdk9 catalytic site to inhibit P-TEFb.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
- Structural insight into the mechanism of stabilization of the 7SK small nuclear RNA by LARP7.Nucleic acids research · 2015Article
- Genetic analysis of the structure and function of 7SK small nuclear ribonucleoprotein (snRNP) in cells.The Journal of biological chemistry · 2014Article
- RNA polymerase II transcription elongation control.Chemical reviews · 2013Review
- HEXIM1 targets a repeated GAUC motif in the riboregulator of transcription 7SK and promotes base pair rearrangements.Nucleic acids research · 2010Article
- Controlling cellular P-TEFb activity by the HIV-1 transcriptional transactivator Tat.PLoS pathogens · 2010Article
- Article
- 7SK snRNA: a noncoding RNA that plays a major role in regulating eukaryotic transcription.Wiley interdisciplinary reviews. RNAArticle
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Eukaryotic transcription by RNA polymerase II is a highly regulated process and divided into three major steps: initiation, elongation, and termination. Each step of transcription is controlled by a number of cellular factors. Positive transcription factor b, P-TEFb, is composed of cyclin-dependent kinase 9 and a regulatory cyclin (T1/T2). P-TEFb promotes transcriptional elongation of RNA polymerase II by using the catalytic function of CDK9 to phosphorylate various substrates during transcription. P-TEFb is inactivated by sequestration in a complex with the Hexim1 protein and 7SK RNA. The structure of this inactive P-TEFb complex and the mechanisms controlling its equilibrium with the active complex are poorly understood. Here, we used a photoactive nucleotide, 4-thioU, to study the interactions between 7SK RNA and Hexim1. We identified a specific cross-link between nucleotide U30 of 7SK RNA and amino acids 210-220 of Hexim1, in the context of both a minimal RNA-binding site and a fully reconstituted 7SK/Hexim1/P-TEFb ribonucleoprotein complex. We show also that a minimal 7SK RNA hairpin comprising nucleotides 24-87 can bind specifically to Hexim1 in vivo. Our results demonstrate directly that the Hexim1 binding site is located in the 24-87 region of 7SK RNA and that the protein residues outside the basic domain of Hexim1 are involved in specific RNA interactions.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.