ArticlePlant physiology2009
Preferential up-regulation of G2/M phase-specific genes by overexpression of the hyperactive form of NtmybA2 lacking its negative regulation domain in tobacco BY-2 cells.
Article in Plant physiology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 33 citations in OpenAlex.
- Overexpression of TgMYB2 from the teak MYB gene family impacts biomass accumulation and secondary cell wall in tobacco plants.Plant molecular biology · 2025Article
- MYB3R-mediated and cell cycle-dependent transcriptional regulation of a tobacco ortholog ofPlant biotechnology (Tokyo, Japan) · 2023Article
- Modelling how plant cell-cycle progression leads to cell size regulation.PLoS computational biology · 2023Article
- Comparative analysis of theFrontiers in plant science · 2023Article
- Genome editing of SlMYB3R3, a cell cycle transcription factor gene of tomato, induces elongated fruit shape.Journal of experimental botany · 2022Article
- Concerted control of the LaRAV1-LaCDKB1;3 module by temperature during dormancy release and reactivation of larch.Tree physiology · 2021Article
- Plant CDKs-Driving the Cell Cycle through Climate Change.Plants (Basel, Switzerland) · 2021Review
- Identification of two tobacco genes encoding MYB3R proteins with repressor function and showing cell cycle-regulated transcript accumulation.Plant biotechnology (Tokyo, Japan) · 2021Article
- TheProceedings of the National Academy of Sciences of the United States of America · 2021Article
- Jasmonate induced alternative splicing responses inPlant direct · 2020Article
- Endoplasmic reticulum retention signaling and transmembrane channel proteins predicted for oilseed ω3 fatty acid desaturase 3 (FAD3) genes.Functional & integrative genomics · 2020Article
- Transcriptome sequencing of Saccharina japonica sporophytes during whole developmental periods reveals regulatory networks underlying alginate and mannitol biosynthesis.BMC genomics · 2019Article
- An R1R2R3 MYB Transcription Factor, MnMYB3R1, Regulates the Polyphenol Oxidase Gene in Mulberry (International journal of molecular sciences · 2019Article
- Evolution of the 3R-MYB Gene Family in Plants.Genome biology and evolution · 2017Article
- Expression patterns of members of the ethylene signaling-related gene families in response to dehydration stresses in cassava.PloS one · 2017Article
- Transcriptional repression by MYB3R proteins regulates plant organ growth.The EMBO journal · 2015Article
- Cosuppression of NtmybA1 and NtmybA2 causes downregulation of G2/M phaseexpressed genes and negatively affects both cell division and expansion in tobacco.Plant signaling & behavior · 2013Article
- Regulating DNA replication in plants.Cold Spring Harbor perspectives in biology · 2012Review
- Enhancement of secondary xylem cell proliferation by Arabidopsis cyclin D overexpression in tobacco plants.Plant cell reports · 2012Article
- Mutations in MYB3R1 and MYB3R4 cause pleiotropic developmental defects and preferential down-regulation of multiple G2/M-specific genes in Arabidopsis.Plant physiology · 2011Article
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Authors and funding
11 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Many G2/M phase-specific genes in plants contain mitosis-specific activator (MSA) elements, which act as G2/M phase-specific enhancers and bind with R1R2R3-Myb transcription factors. Here, we examined the genome-wide effects of NtmybA2 overexpression, one of the R1R2R3-Myb transcription factors in tobacco (Nicotiana tabacum). We used a custom-made 16-K cDNA microarray for comparative transcriptome analysis of transgenic tobacco BY-2 cell lines that overexpress NtmybA2 or its truncated hyperactive form. The microarray was also used to determine the transcript profile during the cell cycle in synchronized cultures of BY-2 cells. Combined microarray data from transgenic lines and synchronized cells revealed that overexpression of the truncated hyperactive form of NtmybA2, but not its full-length form, preferentially up-regulated many G2/M phase-specific genes in BY-2 cells. We determined promoter sequences of several such up-regulated genes and showed that all contain MSA-like motifs in the proximal regions of their promoters. One of the up-regulated genes, NtE2C, encoding for cyclin-specific ubiquitin carrier proteins, contained a single functional MSA-like motif, which specifically controlled the expression of a reporter gene in the G2/M phase in BY-2 cells. Furthermore, a genomic footprint experiment showed that the MSA element in the NtE2C promoter interacted with nuclear proteins in vivo. Therefore, we propose that the transcription of many G2/M phase-specific genes in tobacco is positively regulated by NtmybA2, in most cases through direct binding to the MSA elements.
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